Description
Akt Activity Assay Kit
Akt is a protein kinase that can be activated by insulin and various growth factors and functions in a pathway involving PI3 kinase. Recent evidence suggests that Akt functions to promote cell survival by actively inhibiting apoptosis. The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3as substrate. Phosphorylation of the GSK-3can be analyzed by Western blot analysis using the phospho-GSK-3specific antibody included in the kit. The kit specifically detects Akt1, Akt2, and Akt3 activities, other kinase activities would not be detected.
Key Information | Description |
Product SKU | BN00679 |
Size | 40 assays |
Detection Method | Western blot analysis using anti-Phospho-GSK-3(Ser 21) antibody at 1:1000 dilutions |
Species Reactivity | Mammalian |
Applications | The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3as substrate. |
Features and Benefits |
|
Kit Components |
|
Storage Conditions | -20°C |
Shipping Conditions | Gel Pack |
USAGE | For Research Use Only! Not For Use in Humans. |