Cell-Based ELISA FAQs

1. My kit was left out at room temperature for several days. Can it still be used?

The kit may have diminished activity after an extended period at room temperature, but it is most likely still usable, especially if left out for only a small amount of time. The most temperature-sensitive components in the kit are the standards, HRP-streptavidin, and detection antibody.

2. Why is there a weak signal/no signal detected from my ELISA Genie Cell-Based ELISA?

This may due to a number of reasons. The possible causes and their solutions are listed below:

Possible Cause Solution

Storage and Expiration

Read Manual for storage condition and expiration.

Antibody Dilution

Dilute antibody 1:100 with Antibody Diluent.

Low Cell Number

Seed more cells

Cells Detach from Well-Bottoms

Add solutions slowly from the side wall of the wells. Use Poly-Lysine to coat the plate period seeding the cells, if necessary.

3. Why is their high background noise from my ELISA Genie Cell-Based ELISA?

This may due to a number of reasons. The possible causes and their solutions are listed below:

Possible Cause Solution

Washing

Remove Wash Buffer completely

High Cell Number

Reduce the amount of seeded cells.

4. Why may there be variation in my sample duplicate results when performing an ELISA Genie Cell-Based ELISA?

This may due to a number of reasons. The possible causes and their solutions are listed below:

Possible Cause Solution

Pipetting

Check and/or calibrate pipettes.

Washing

Remove Wash Buffer completely.

Cells Detach from Well-Bottoms

Add solutions slowly from the side wall of the wells. Use Poly-L-Lysine to coat the plate period seeding the cells, if necessary.