Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Size Exclusion Chromatography (SEC) is considered one of the best methods for isolating and purifying exosomes and extracellular vesicles (EVs) from different matrices. This technique is very efficient for separating EVs from the circulating proteins without affecting the original shape and functionality of the vesicles. ExoGenie Chromatography Columns is a SEC column designed for isolating EVs in a fast and easy way.

Figure 1: 3. Matching of EV quantity and total protein content eluted in each single fraction. SEC Columns was rinsed with 1 ml of human plasma, 24 fractions (500 μl each one) have been collected and analyzed by ELISA assay and by BCA test for determining respectively vesicle and total protein content. EVs are eluted in fractions 6-11 (turnaround time approximately 15 min), whereas plasma circulating proteins corresponded to the fractions 14-24.

Figure 2: A. EV elution peak. ExoGenie vs NTA analysis. B. EV elution vs circulating protein elution. NTA analysis compared to protein BCA test. C. ExoGenie analysis compared to protein BCA test.