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HMG-CoA Reductase Activity/Inhibitor Screening Kit (Colorimetric) (BN00816)

SKU:
BN00816
Product type:
Cellular Assay
  • Cell Biology Assays HMG-CoA Reductase Activity/Inhibitor Screening Kit Colorimetric BN00816
  • Cell Biology Assays HMG-CoA Reductase Activity/Inhibitor Screening Kit Colorimetric BN00816
€884

Description

ELISA Kit Technical ManualMSDS

HMG-CoA Reductase Activity/Inhibitor Screening Kit (Colorimetric)

HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl-CoA reductase or HMGR) (EC 1.1.1.34) is the rate-controlling enzyme of the mevalonate pathway, the metabolic pathway that produces cholesterol from acetyl-CoA. In an NADPH-dependent reaction, HMG-CoA reductase reduces HMG-CoA to generate mevalonate and CoA. The enzyme is target of a group of cholesterol-lowering drugs known as statins. Inhibition of HMG-CoA reductase induces expression of LDL receptors in the liver, which lowers plasma concentration of cholesterol. Assay Genie's HMG-CoA Reductase Activity Assay Kit measures utilization of NADPH, which can be measured by the decrease of absorbance (OD 340 nm). The kit is suitable for measuring activity of purified enzyme and screening inhibitors/activators of HMG-CoA reductase. The limit of detection is below 0.05 mU.

(a)

(b)

Figure: (a) HMG-CoA Reductase (Positive Control) activity. (b) Inhibition of HMG-CoA Reductase activity by Atorvastatin (100 µM) and Pravastatin (10 ng/µl).

ND: Not Detectable. Assay was performed according to kit protocol.

Key Information Description

Product SKU

BN00816

Size

100 assays

Detection Method

Absorbance (OD 340 nm)

Applications

  • Measurement of HMG-CoA reductase activity
  • Screening/studying/characterizing HMG-CoA reductase inhibitors/activators

Features and Benefits

  • Simple, rapid & convenient
  • Limit of detection: below 0.05 mU
  • Includes inhibitor (Atorvastatin)

Kit Components

  • HMG-CoA Reductase Assay Buffer
  • HMG-CoA Reductase
  • HMG-CoA
  • NADPH
  • Inhibitor (Atorvastatin, 10 mM)

Storage Conditions

-80°C

Shipping Conditions

Dry Ice

USAGE

For Research Use Only! Not For Use in Humans