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Myoglobin Monoclonal Antibody [PAT6E10] (CPAB0450)

SKU:
CPAB0450
Product type:
Antibody
Reactivity:
Other bodies
Host Species:
Mouse
Isotype:
IgG1
Clone:
PAT6E10
Application:
WB
Application:
ELISA
€180
Frequently bought together:

Description

Technical Manual

Myoglobin Monoclonal Antibody [PAT6E10] (CPAB0450)

Product Name:Myoglobin Antibody
Product Sku:CPAB0450
Size:5μg
Host Species:Mouse
Purification Method:Myoglobin antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Isotype:IgG1
Background:Myoglobin is a member of the globin superfamily and can be found in skeletal and cardiac muscles. It is a haemoprotein that contributes to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. Myoglobin has a single-chain globular structure of 153 amino acids, containing a heme prosthetic group (iron-containing porphyrin) in the core around which the remaining apoprotein folds. Myoglobin has 8 alpha helices and a hydrophobic core. Myoglobin’s molecular weight is 16.7 kDa, and it is the primary oxygen-carrying pigment of muscle tissues. The binding of oxygen in myoglobin is different from the cooperative oxygen binding in hemoglobin, since positive collaboration is a property of multimeric/oligomeric proteins only. Instead, the binding of oxygen by myoglobin is uninfluenced by the oxygen pressure in the surrounding tissue. Myoglobin is found in Type I muscle, Type II A and Type II B, but it is mostly deemed that myoglobin is not found in smooth muscle. Myoglobin is discharged from damaged muscle tissue (rhabdomyolysis), which contains very high concentrations of myoglobin. Even though the released myoglobin is filtered by the kidneys, it is toxic to the renal tubular epithelium and thus may cause acute renal failure.
Immunogen:Anti-human Myoglobin, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Myoglobin amino acids 1-154 purified from Ecoli.
Synonyms:Myoglobin, MB, PVALB, MGC13548.
Clone:PAT6E10.
Reactivity:Other bodies
Applications:Western Blot, ELISA
Storage Buffer:For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles.