A high sensitivity ELISA reaches a lower limit of detection than a standard kit for the same analyte — typically single-digit picograms per millilitre rather than tens or hundreds. That comes from three things: higher-affinity matched antibody pairs, an amplified detection step (biotin–streptavidin–HRP rather than a directly conjugated antibody), and a standard curve recalibrated across a lower concentration range so the bottom of the curve is properly resolved.

It depends on the analyte. The most sensitive kits in the range reach around 0.19 pg/mL, and many sit between 0.3 and 5 pg/mL. Each product page lists the kit’s own sensitivity and detection range — check those figures against the concentrations you expect in your matrix before ordering.

Most kits are validated in serum, plasma, cell culture supernatant, cell lysate, tissue homogenate and other biological fluids. Validated matrices are listed per kit, because a kit validated in serum will not necessarily perform identically in urine or CSF without a matrix check.

Use a high sensitivity kit when your expected analyte concentration is at or below the standard kit’s lower limit of quantification, when your sample volume is limited so you cannot concentrate the sample, or when you need to resolve small fold-changes near baseline. If your analyte is abundant, a standard kit gives you a wider upper range and fewer dilution steps.

Yes — several kits in the range are supplied in a small-sample-volume format, designed for cases where only a few microlitres of serum or plasma are available, such as serial bleeds from mouse studies. These are listed alongside the standard-format kits in the catalogue.

Human, mouse and rat make up the bulk of the range, with additional kits for canine, monkey and bovine samples. Use the reactivity filter on the high sensitivity category page to see what is available for your model.

Intra- and inter-assay coefficients of variation are held below 10%. Each batch is checked for lot-to-lot consistency so results remain comparable across timepoints and between studies, which matters when you are tracking small changes at the low end of the curve.

Get in touch. If a high sensitivity kit for your target isn’t in the catalogue, our team can advise on the closest available assay or scope a custom ELISA development project. Email sales@assaygenie.com with your target, species and expected concentration range.