null

Anti-Lin28 Antibody (CAB9627)

SKU:
CAB9627
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Synonyms:
CSDD1
LIN-28
LIN28
ZCCHC1
lin-28A
Research Area:
Epigenetics and Nuclear Signaling
€139 - €419
Frequently bought together:

Description

Product Name:Lin28 Rabbit mAb
Product Code:CAB9627
Size:20uL, 50uL, 100uL
Synonyms:CSDD1, LIN-28, LIN28, ZCCHC1, lin-28A
Applications:WB, IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human Lin28
Applications:WB, IF
Recommended Dilutions:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse testis, Rat testis
Immunogen:A synthesized peptide derived from human Lin28
Purification Method:Affinity purification
Storage:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:79727
Uniprot:Q9H9Z2
Calculated MW:26kDa
Observed MW:26KDa
UniProt Protein Function:LIN28A: Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression. Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let- 7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre- let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Monomer. During skeletal muscle differentiation, associated with translation initiation complexes in the polysomal compartment. Directly interacts with EIF3S2. Interaction with NCL is RNA-dependent. Interacts with ZCCHC11/TUT4. Can be negatively regulated by the interaction of microRNAs miR-125a and miR-125b with at least two miRNA responsive elements (miREs) in the 3'-UTR of this gene. These interactions may reduce both translation efficiency and mRNA abundance. Negatively regulated by retinoic acid. Expressed in embryonic stem cells (ES cells), placenta and testis. Belongs to the lin-28 family.
UniProt Protein Details:

Protein type:Nucleolus; RNA-binding; Translation

Chromosomal Location of Human Ortholog: 1p36.11

Cellular Component: cytoplasm; cytosol; nucleus; stress granule

Molecular Function:protein binding; RNA binding

Biological Process: pre-microRNA processing; RNA 3'-end processing; somatic stem cell maintenance; stem cell maintenance

NCBI Summary:This gene encodes a LIN-28 family RNA-binding protein that acts as a posttranscriptional regulator of genes involved in developmental timing and self-renewal in embryonic stem cells. The encoded protein functions through direct interaction with target mRNAs and by disrupting the maturation of certain miRNAs involved in embryonic development. This protein prevents the terminal processing of the LET7 family of microRNAs which are major regulators of cellular growth and differentiation. Aberrant expression of this gene is associated with cancer progression in multiple tissues. [provided by RefSeq, Sep 2015]
UniProt Code:Q9H9Z2
NCBI GenInfo Identifier:74752750
NCBI Gene ID:79727
NCBI Accession:Q9H9Z2.1
UniProt Related Accession:Q9H9Z2
Molecular Weight:22,743 Da
NCBI Full Name:Protein lin-28 homolog A
NCBI Synonym Full Names:lin-28 homolog A
NCBI Official Symbol:LIN28A  
NCBI Official Synonym Symbols:CSDD1; LIN28; LIN-28; ZCCHC1; lin-28A  
NCBI Protein Information:protein lin-28 homolog A
UniProt Protein Name:Protein lin-28 homolog A
UniProt Synonym Protein Names:Zinc finger CCHC domain-containing protein 1
Protein Family:Protein
UniProt Gene Name:LIN28A  
UniProt Entry Name:LN28A_HUMAN

Related Products