E.Coli GLSA1 Recombinant Protein (RPPB1705)
- SKU:
- RPPB1705
- Product Type:
- Recombinant Protein
- Species:
- Escherichia coli
- Uniprot:
- P77454
- Research Area:
- Microbiology
Frequently bought together:
Description
| Product Name: | E.Coli GLSA1 Recombinant Protein |
| Product Code: | RPPB1705 |
| Size: | 20µg |
| Species: | E.Coli |
| Target: | GLSA1 |
| Synonyms: | Glutaminase 1, ybaS. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GLSA1 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MLDANKLQQA VDQAYTQFHS LNGGQNADYI PFLANVPGQL AAVAIVTCDG NVYSAGDSDY RFALESISKV CTLALALEDV GPQAVQDKIG ADPTGLPFNS VIALELHGGK PLSPLVNAGA IATTSLINAE NVEQRWQRIL HIQQQLAGEQ VALSDEVNQS EQTTNFHNRA IAWLLYSAGY LYCDAMEACD VYTRQCSTLL NTIELATLGA TLAAGGVNPL THKRVLQADN VPYILAEMMM EGLYGRSGDW AYRVGLPGKS GVGGGILAVV PGVMGIAAFS PPLDEDGNSV RGQKMVASVA KQLGYNVFKG |
GlsA1 is a member of the great superfamily of serine-dependent beta-lactamases and penicillin-binding proteins which exist almost in every bacteria and eukaryotes and catalyze the hydrolytic deamidation of l-glutamine to l-glutamate and free NH4+.
GLSA1 E.Coli Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 330 amino acids (1-310a.a.) and having a molecular mass of 35.0kDa. The GLSA1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | |
| UniProt Protein Details: | |
| NCBI Summary: | Two glutaminases have been purified and biochemically characterized from Escherichia coli B. ybaS might contribute to acid resistance (Brown, 2008). [More information is available at EcoGene: EG13247]. YbaS is a glutaminase that is highly selective for L-glutamine. [More information is available at EcoCyc: G6261]. |
| UniProt Code: | P77454 |
| NCBI GenInfo Identifier: | 16128469 |
| NCBI Gene ID: | 946187 |
| NCBI Accession: | NP_415018.1 |
| UniProt Secondary Accession: | P77454,Q2MBU2 |
| UniProt Related Accession: | P77454 |
| Molecular Weight: | 32,903 Da |
| NCBI Full Name: | glutaminase 1 |
| NCBI Synonym Full Names: | |
| NCBI Official Symbol: | glsA�� |
| NCBI Official Synonym Symbols: | ECK0479; glsA1; JW0474; ybaS�� |
| NCBI Protein Information: | glutaminase 1 |
| UniProt Protein Name: | Glutaminase 1 |
| UniProt Synonym Protein Names: | |
| Protein Family: | Glutaminase |
| UniProt Gene Name: | glsA1�� |
| UniProt Entry Name: | GLSA1_ECOLI |
