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Low & Ultra-Low Endotoxin In Vivo Antibodies

Q: Are isotype controls available for these clones?

Yes, matched isotype control antibodies are available at the same endotoxin tier for use as your negative control in dosing studies.

Low & Ultra-Low Endotoxin In Vivo Antibodies | Assay Genie

In Vivo Antibodies

Low & Ultra-Low Endotoxin In Vivo Antibodies

Functional-grade antibodies purified and tested to strict endotoxin thresholds — built for sensitive preclinical mouse studies where LPS contamination can confound your results.

Shop Low Endotoxin Shop Ultra-Low Endotoxin

≤1.0 EU/mg Low ≤0.5 EU/mg Ultra-Low IDEXX Impact tested >95% purity

Anti-PD-1 [RMP1-14]

Anti-CTLA-4 [9H10]

Anti-IFNAR-1 [MAR1-5A3]

≤1.0

EU/mg Low Endotoxin

≤0.5

EU/mg Ultra-Low Endotoxin

>95%

Purity by SEC

IDEXX

Impact Pathogen-Tested

Why Endotoxin Level Matters

Even trace lipopolysaccharide (LPS) contamination can trigger innate immune activation in vivo, confounding cytokine readouts, tumor growth kinetics, and survival studies — especially in immune-oncology and inflammation models.

Two endotoxin tiers

Low Endotoxin (≤1.0 EU/mg) for routine in vivo dosing, and Ultra-Low Endotoxin (≤0.5 EU/mg) for the most LPS-sensitive models — choose the tier your study design demands.

IDEXX Impact pathogen-tested

Every lot is screened against the IDEXX Impact panel to exclude murine pathogen contamination before it ever reaches your animals.

Azide-free, carrier-free formulation

Formulated without sodium azide or carrier proteins (BSA/gelatin) that could otherwise interfere with in vivo dosing or introduce unwanted immunogenicity.

In vivo-validated clones

Clones are selected and functionally validated for in vivo blocking, depleting, or agonist activity — not simply repurposed from flow cytometry panels.

Low vs. Ultra-Low Endotoxin Antibody Specifications

Specifications	Low Endotoxin	Ultra-Low Endotoxin
Binding validation determined by WB, FC or ELISA	Yes	Yes
Endotoxin level determined by the LAL method	≤ 1.0 EU/mg	≤ 0.5 EU/mg
Antibody aggregation screening by analytical SEC	≥ 95% monomer	≥ 95% monomer
Purity by SDS-PAGE	≥ 95%	≥ 95%
Murine pathogen screening	Not applicable	Pathogen tested (IMPACT1)
No preservatives, stabilizers, or carrier protein — sterile PBS pH 7.2, >5mg/mL	Yes	Yes

Built for Tumor and Immune-Oncology Mouse Models

Low and ultra-low endotoxin formats are purpose-built for syngeneic tumor models (CT26, MC38, B16F10) and other immune-oncology studies where checkpoint blockade, cell depletion, or cytokine-pathway antibodies are dosed systemically over multiple weeks. Minimizing endotoxin load reduces the risk of non-specific innate immune activation that can mask or exaggerate a treatment effect.

Generation of tumor mouse models workflow for in vivo antibody dosing studies

Typical workflow: syngeneic tumor cell implantation, randomization, and systemic antibody dosing in an in vivo efficacy study.

Anti-PD-1 [RMP1-14] — Quality Control Summary

Representative lot-release data for a Low Endotoxin in vivo antibody, confirming endotoxin level, purity, and integrity ahead of dosing.

Endotoxin0.269 EU/mg — passes ≤1.0 EU/mg Low Endotoxin threshold

Purity (SEC)>95% monomeric IgG, no high-MW aggregates

SDS-PAGESingle band under reducing and non-reducing conditions at expected MW

FormatAzide-free, carrier-free, PBS formulation

Anti-PD-1 [RMP1-14] quality control data: endotoxin analysis, chromatography, and reducing/non-reducing SDS-PAGE

A) Endotoxin analysis. B) Size-exclusion chromatography. C) Non-reducing SDS-PAGE. D) Reducing SDS-PAGE.

IDEXX Impact Pathogen Screening

Every lot in the Ultra-Low Endotoxin tier is screened against the IDEXX Impact panel — a comprehensive murine pathogen screen — before it ships, to keep your animal colony protected.

Tested Mouse Pathogens
Mycoplasma sp.	Mouse adenovirus 1 (MAV1)	Lactate dehydrogenase-elevating virus (LDEV)
Mycoplasma pulmonis	Mouse adenovirus 2 (MAV2)	Mouse kidney parvovirus (MKPV)
Sendai virus	Murine norovirus (MNV)	Hantaan virus
Mouse hepatitis virus (MHV)	Reovirus 3 (REO3)	Mouse cytomegalovirus (mCMV)
Pneumonia virus of mice (PVM)	Mouse rotavirus (EDIM)	K virus
Minute virus of mice (MVM)	Ectromelia virus	Mouse thymic virus (MTV)
Mouse parvovirus (MPV)	Polyomavirus	Corynebacterium bovis
Theiler's murine encephalomyelitis (TMEV)	Lymphocytic choriomeningitis virus (LCMV)	Corynebacterium sp.

Explore the Full In Vivo Range

Need a different format? Browse our bispecific and biosimilar in vivo antibody lines.

Bispecific

Bispecific In Vivo Antibodies

Dual-targeting in vivo antibodies for simultaneous engagement of two antigens in a single molecule.

Single-molecule dual targeting
Functional-grade, in vivo validated
Low endotoxin formats available

View Bispecific range

Biosimilar

VivoGenie® Biosimilar Antibodies

Cost-effective biosimilar alternatives to leading reference in vivo antibodies, at a fraction of the cost.

Matched to reference clone activity
Significant cost savings vs. reference
Bulk quantities available

View VivoGenie® range

Recent Citations

Assay Genie in vivo antibodies referenced in published research.

Journal of Immunology

Study utilizing anti-PD-1 checkpoint blockade antibodies to characterize T-cell exhaustion dynamics in a syngeneic tumor model.

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Cancer Immunology Research

Anti-CTLA-4 in vivo antibody used to evaluate combination checkpoint blockade efficacy and tolerability in CT26 colorectal carcinoma models.

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Frontiers in Immunology

Depleting antibodies applied to dissect the contribution of specific immune cell subsets to anti-tumor immunity in vivo.

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Nature Communications

Cytokine receptor-blocking in vivo antibodies used to interrogate interferon signaling pathways in a chronic inflammation model.

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Journal of Experimental Medicine

Low endotoxin in vivo antibodies selected specifically to avoid confounding innate immune activation during longitudinal dosing studies.

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OncoImmunology

Isotype-matched control antibodies used alongside targeted in vivo antibodies to validate treatment-specific effects in tumor growth assays.

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Browse the Citations Hub

What Researchers Are Saying

“

Switching to the low endotoxin format eliminated the background cytokine activation we were seeing in our vehicle controls — a much cleaner readout for our tumor model.

Preclinical Research Scientist, Academic Cancer Center

Popular In Vivo mAbs

Frequently ordered low and ultra-low endotoxin in vivo antibodies.

IVMB0037

Anti-Mouse PD-1 (CD279) [RMP1-14]

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IVMB0047

Anti-Mouse CTLA-4 [9H10]

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IVMB0202

Anti-Mouse IFNAR-1 [MAR1-5A3]

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IVMB0198

Rat IgG2a Isotype Control

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Why Choose Assay Genie

🚚

Fast Delivery

Most low and ultra-low endotoxin formats ship within days from stock.

🎓

PhD-Level Support

Talk to our scientists about clone and endotoxin-tier selection for your study design.

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Functional-Grade Quality

Every lot is endotoxin-tested and QC-verified before it ships.

Resources

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Technical Manuals

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MSDS Sheets

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Citations Hub

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Ask a Scientist

Frequently Asked Questions

What's the difference between Low and Ultra-Low Endotoxin?

Low Endotoxin antibodies test at ≤1.0 EU/mg, suitable for most in vivo dosing studies. Ultra-Low Endotoxin antibodies test at ≤0.5 EU/mg, intended for the most LPS-sensitive models (e.g. sepsis, acute inflammation) where even trace endotoxin can confound results.

What does "functional grade" mean for an in vivo antibody?

It means the antibody has been specifically formulated and QC-tested for in vivo use — low endotoxin, azide-free, carrier-free — rather than simply repurposed from a flow cytometry or ELISA product line.

Are isotype controls available for these clones?

Yes, matched isotype control antibodies (e.g. Rat IgG2a) are available at the same endotoxin tier for use as your negative control in dosing studies.

What is IDEXX Impact pathogen testing?

IDEXX Impact is a comprehensive murine pathogen screening panel. Testing lots against this panel helps ensure your in vivo antibody preparation won't introduce unwanted pathogens into your animal colony.

How should I store these antibodies?

Store at 4°C for short-term use and avoid repeated freeze-thaw cycles; consult the technical manual for your specific product for long-term storage guidance.

Do you offer bispecific or biosimilar in vivo formats?

Yes — see our Bispecific and VivoGenie® Biosimilar in vivo antibody ranges.

Can't find the target or clone you need?

Contact our scientific support team — we can help source or custom-develop an in vivo antibody against your target of interest.

Ready to Start Your In Vivo Study?

Browse the full low and ultra-low endotoxin range, or talk to our scientists about your specific model.

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