Anti-NPHS1 Antibody (CAB3048)

Antibody Name:	Anti-NPHS1 Antibody
Antibody SKU:	CAB3048
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human NPHS1

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse kidney, mouse brain, rat brain

Immunogen:	A synthetic peptide of human NPHS1
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	4868
Uniprot:	O60500
Cellular Location:	Cell membrane, Single-pass type I membrane protein
Calculated MW:	130kDa/134kDa
Observed MW:	130kDa

Synonyms:

NPHS1, CNF, NPHN, nephrin

Background:

This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.

UniProt Protein Function:	NPHS1: Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. Interacts with CD2AP (via C-terminal domain). Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4. Interacts with DDN; the interaction is direct. Self-associates (via the Ig-like domains). Also interacts (via the Ig-like domains) with KIRREL/NEPH1 and KIRREL2; the interaction with KIRREL is dependent on KIRREL glycosylation. Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1. Interacts with NPHS2. Specifically expressed in podocytes of kidney glomeruli. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Immunoglobulin superfamily; Membrane protein, integral; Cell adhesion Chromosomal Location of Human Ortholog: 19q13.1 Cellular Component: integral to plasma membrane; plasma membrane Molecular Function:myosin binding; protein binding Biological Process: excretion; glomerular basement membrane development Disease: Nephrotic Syndrome, Type 1
NCBI Summary:	This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]
UniProt Code:	O60500
NCBI GenInfo Identifier:	20177993
NCBI Gene ID:	4868
NCBI Accession:	O60500.1
UniProt Secondary Accession:	O60500,A6NDH2, C3RX61,
UniProt Related Accession:	O60500
Molecular Weight:	130,546 Da
NCBI Full Name:	Nephrin
NCBI Synonym Full Names:	NPHS1, nephrin
NCBI Official Symbol:	NPHS1
NCBI Official Synonym Symbols:	CNF; NPHN; nephrin
NCBI Protein Information:	nephrin
UniProt Protein Name:	Nephrin
UniProt Synonym Protein Names:	Renal glomerulus-specific cell adhesion receptor
Protein Family:	Nephrin
UniProt Gene Name:	NPHS1
UniProt Entry Name:	NPHN_HUMAN

	Western blot analysis of extracts of mouse kidney, using NPHS1 antibody (CAB3048) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Western blot analysis of extracts of various cell lines, using NPHS1 antibody (CAB3048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse kidney using NPHS1 antibody (CAB3048) (40x lens).
	Immunofluorescence analysis of mouse kidney cells using NPHS1 antibody (CAB3048) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-NPHS1 Antibody (CAB12467)	Human Nephrin ELISA Kit
NPHS1 Antibody (PACO04459)	Human NPHN (Nephrin) CLIA Kit (HUES01049)
	Human Nephrin (NPHS1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)