Acute Kidney Injury (AKI) 5-Plex Immunoassay Panel

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ELISA Kit Technical Manual

Acute Kidney Injury (AKI) 5-Plex Immunoassay Panel 


IP-10, TIM-1, OPN, NGAL, Cystatin C

Species Reactivity:


Assay Type:


Detection Method:

Flow Cytometry

Assay Time:

2 hours

Product Overview

This GeniePlex GeniePlex Acute Kidney Injury (AKI) 5-Plex Immunoassay Panel is a bead-based Multiplex Immunoassay kit that enables the simultaneous and quantitative detection of the following analytes IP-10, TIM-1, OPN, NGAL, Cystatin C on almost any flow cytometer in a wide variety of samples such as Serum, Plasma, Cell culture supernatant, Cell lysates, Tissue lysates and other samples types.

GeniePlex uses a unique mix of antibody-coated encoded microparticles providing an ultra-sensitive technology for the quantitation of analytes.

At ELISA Genie we understand the need for quantitative reproducible results! Therefore, we have developed a simplified protocol meaning you can easily quantify 4 analytes in as little as 15ul of sample all in 32-test or 96-test filter plate format for rapid analysis.

As our beads and standards come pre-mixed, simply add your pre-defined bead panel to the wells followed by the pre-mixed standards or samples. After an incubation and wash step, add Biotinylated detection antibodies and repeat incubation & wash. Next step is the addition of Streptavidin-PE which binds the Biotinylated detection antibody. After a final incubation & washing step, Reading buffer is added and the assay analysed on a flow cytometer with either a 488nm laser or 488nm and 633/640nm lasers. The concentration of the analytes in the samples is determined by comparing the fluorescent signals to a standard curve generated from a serial dilution of a known concentration of the analyte.

Product Information

Reactivity: Human
Analytes: IP-10, TIM-1, OPN, NGAL, Cystatin C
1x Ab-conjugated beads (premixed): S4P3 - anti-IP-10; S4P6 - anti-human TIM-1; S4P7 - anti-human OPN; S5P2 - anti-human NGAL, S5P9 - anti-human Cystatin C
2x Biotin-detection Ab (premixed): Biotin anti-human IP-10; Bioin - anti-human TIM-1; Biotin - anti-human OPN; Biotin - anti-human NGAL, Biotin - anti-human Cystatin C
Buffers: 2x NR dAb Diluent. One vial containing 1.5 mL of biotin detection antibody diluent
Lyophilized Standard Mix: Lyophilized Standard Mix-Human T Helper Cytokine 4-Plex Panel 2. One vial containing lyophilized recombinant IFN-Gamma, IL-4, IL-10, TNF-Alpha.
Sample types: Sample types: Cell culture supernatant, serum, plasma, bodily fluid and tissue/cell lysate
Sensitivity (LODs): Cystatin C, < 20 pg/mL; IP-10, < 3 pg/mL; NGAL, < 50 pg/mL; OPN, < 5 pg/mL; TIM-1, < 5 pg/mL.
LLOQs: Cystatin C, < 40 pg/mL; IP-10, < 10 pg/mL; NGAL, < 100 pg/mL; OPN, < 10 pg/mL; TIM-1, < 10 pg/mL
ULOQs: Cystatin C, > 20,000 pg/mL; IP-10, > 2,000 pg/mL; NGAL, > 50,000 pg/mL;  OPN, > 5,000 pg/mL; TIM-1, > 5,000 pg/mL
Standard dose recovery: Standard dose recovery: 70-130%
Intra-assay CV: Intra-assay CV: < 10%
Inter-assay CV: Inter-assay CV: < 20%
Cross-reactivity of analytes in the Panel: Cross-reactivity of analytes in the Panel: Negligible
Sample volume: Sample volume: 15 µL/test
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