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Anti-ALCAM Antibody (CAB2218)

SKU:
CAB2218
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology
  • Immunology Antibodies 2 Anti-ALCAM Antibody CAB2218
  • Immunology Antibodies 2 Anti-ALCAM Antibody CAB2218
€139 - €419
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Description

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Antibody Name:Anti-ALCAM Antibody
Antibody SKU:CAB2218
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 28-180 of human ALCAM (NP_001618.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:HepG2, A375, U-87MG, A-549, BT-474, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 28-180 of human ALCAM (NP_001618.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:WYTV NSAY GDTI IIPC RLDV PQNL MFGK WKYE KPDG SPVF IAFR SSTK KSVQ YDDV PEYK DRLN LSEN YTLS ISNA RISD EKRF VCML VTED NVFE APTI VKVF KQPS KPEI VSKA LFLE TEQL KKLG DCIS EDSY PDGN ITWY RNGK VLHP L
Gene ID:214
Uniprot:Q13740
Cellular Location:Membrane, Secreted, Single-pass type I membrane protein
Calculated MW:15kDa/33kDa/63kDa/65kDa
Observed MW:105kDa
Synonyms:ALCAM, CD166, MEMD
Background:This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.
UniProt Protein Function:ALCAM: Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and homophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 3q13.1

Cellular Component: focal adhesion; cell soma; axon; integral to membrane; external side of plasma membrane

Molecular Function:protein binding; receptor binding

Biological Process: axon guidance; motor axon guidance; signal transduction; cell adhesion

NCBI Summary:This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found. [provided by RefSeq, Aug 2011]
UniProt Code:Q13740
NCBI GenInfo Identifier:118572629
NCBI Gene ID:214
NCBI Accession:Q13740.2
UniProt Secondary Accession:Q13740,O60892, Q1HGM8, Q1HGM9, Q6PEY4, Q6ZS95, B2RNS3 B4DTU0,
UniProt Related Accession:Q13740
Molecular Weight:33,878 Da
NCBI Full Name:CD166 antigen
NCBI Synonym Full Names:activated leukocyte cell adhesion molecule
NCBI Official Symbol:ALCAM  
NCBI Official Synonym Symbols:MEMD; CD166  
NCBI Protein Information:CD166 antigen
UniProt Protein Name:CD166 antigen
UniProt Synonym Protein Names:Activated leukocyte cell adhesion molecule; CD_antigen: CD166
Protein Family:CD166 antigen
UniProt Gene Name:ALCAM  
UniProt Entry Name:CD166_HUMAN
Anti-ALCAM Antibody (CAB2218)Western blot analysis of extracts of various cell lines, using ALCAM antibody (CAB2218) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-ALCAM Antibody (CAB2218)Western blot analysis of extracts of Mouse liver, using ALCAM antibody (CAB2218) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

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