alpha-Ketoglutarate (alpha-KG) is a key intermediate in the Krebs cycle, coming after isocitrate and before succinyl CoA. Anaplerotic reactions replenish the cycle by synthesizing alpha-KG from transamination of glutamate, or through the action of glutamate dehydrogenase. alpha-KG is an important nitrogen transporter. Being a key intermediate, it is one of the organic acids measured in newborns as an indicator of inborn errors of metabolism. Assay Genie's alpha-Ketoglutarate Assay Kit provides a simple, sensitive and rapid means for quantifying alpha-KG in a variety of samples. In the assay, alpha-ketoglutarate is transaminated with the generation of pyruvate which is utilized to convert a nearly colorless probe to both color (Abs»max = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The alpha-Ketoglutarate Assay Kit is useful for detecting alpha-ketoglutarate in the range of 0.01 to 10 nmoles.
|Detection Method:||Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)|
|Applications:||The alpha-Ketoglutarate Assay Kit is useful for detecting alpha-ketoglutarate in the range of 0.01 to 10 nmoles.|
|Features & Benefits:||Simple procedure; takes ~ 30 minutes to complete the assay Fast and convenient Highly sensitive assays for measuring alpha-Ketaglutarate levels in biological samples|
|Kit Components:||alpha-KG Assay Buffer, alpha-KG OxiRed Probe (in DMSO), alpha-KG Converting Enzyme, alpha-KG Development Enzyme Mix, alpha-KG Standard (10 umol)|
|Shipping Conditions:||Gel Pack|
|USAGE:||For Research Use Only! Not For Use in Humans.|