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Anti-GPBAR1 Antibody (CAB20778)

SKU:
CAB20778
Product Type:
Antibody
Antibody Type:
Secondary Antibody
Host Species:
Rabbit
Isotype:
Horseradish peroxidase conjugated IgG
  • Immunofluorescence analysis of HCT116 cells using GPBAR1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using GPBAR1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of 293T cells using GPBAR1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Rat liver, using GPBAR1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
€139 - €469
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Description

Antibody Name:Anti-GPBAR1 Antibody (CAB20778)
Antibody SKU:CAB20778
Antibody Size:50µL, 100µL
Application:Western blotting, Immunofluorescence
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 231-330 of human GPBAR1 (NP_733800.1).
Application:Western blotting, Immunofluorescence
Recommended Dilution:WB 1:500 - 1:2000
IF 1:50 - 1:200
Reactivity:Human, Rat
Positive Samples:Rat liver
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 231-330 of human GPBAR1 (NP_733800.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LLFG LCWG PYVA TLLL SVLA YEQR PPLG PGTL LSLL SLGS ASAA AVPV AMGL GDQR YTAP WRAA AQRC LQGL WGRA SRDS PGPS IAYH PSSQ SSVD LDLN
Cellular Location:
Calculated MW:35kDa
Observed MW:35KDa
Synonyms:BG37, TGR5, M-BAR, GPCR19, GPR131
Background:This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein.
Immunofluorescence analysis of HCT116 cells using GPBAR1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of HCT116 cells using GPBAR1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HepG2 cells using GPBAR1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of HepG2 cells using GPBAR1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of 293T cells using GPBAR1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunofluorescence analysis of 293T cells using GPBAR1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of Rat liver, using GPBAR1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Western blot analysis of extracts of Rat liver, using GPBAR1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

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