Basic RNA extraction kit (Pre-isolated exosomes, 25 reactions) (BN00082)

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BN00082
  • Basic RNA extraction kit (Pre-isolated exosomes) (BN00082)
  • Basic RNA extraction kit (Pre-isolated exosomes) (BN00082)
€1,299

Description

ELISA Kit Technical ManualMSDS

Basic RNA extraction kit (Pre-isolated exosomes)

Exosomes are small endosome derived lipid nanoparticles (50-120 nm) actively secreted by exocytosis by most living cells. Exosome release occurs either constitutively or upon induction, under both normal and pathological conditions, in a dynamic, regulated and functionally relevant manner. Both the amount and molecular composition of released exosomes depend on the state of a parent cell. Exosomes have been isolated from diverse cell lines (hematopoietic cells, tumor lines, primary cultures, and virus infected cells) as well as from biological fluids in particular blood (e.g. serum and plasma from cancer patients) and other body fluids (broncho alveolar lavage fluid, pleural effusions, synovial fluid, urine, amniotic fluid, semen, saliva etc). Exosomes have pleiotropic physiological and pathological functions and an emerging role in diverse pathological conditions such as cancer, infectious and neurodegenerative diseases. Exosomes shuttle functional RNA molecules in the target cell. Increasing evidence suggests a role for exosome-derived miRNAs in the development and/or progression of specific human diseases. Pathogenic miRNAs might be exploited as novel therapeutic targets or disease biomarkers in complex diseases, including cancer. In fact, miRNAs seem to play critical roles as transcriptional and post-transcriptional regulators of epigenetic mechanisms and cell processes and have been linked to the etiology, progression and prognosis of cancer. Similar miRNA expression patterns between tumor tissue samples and circulating exosomes have been observed. Basic RNA extraction kit allows total RNA (miRNA + mRNAs) extraction from pre-isolated exosomes pre-isolated via different methods including ultracentrifugation, chemical precipitation, immunocapture, size-chromatography etc). *RNA basic kit does not contain immunobeads for exosome isolation. Pre-isolated exosomes are lysed with an optimized lysis buffer and total RNA is purified using spin columns with a fast and user-friendly process. Eluted RNA can be used for downstream analyses or stored at -80°C. Exosome standards for positive control are also included in the kit. All our kits guarantee high specificity for exosomal RNA and high yield of total RNA (including small RNAs) than similar products.

Figure: 1) Expected Nanodrop profile for RNA extracted from immunocaptured exosomes (100 μl of human plasma). Yield = 8.4 ng/μl; A 260/280 =1.6; A 260/230 = 1.5. 2) Nanodrop quantification of total RNA yield. 3) miRNA 21 amplification by qPCR.

Key Information Description

Detection Method

Pre-isolated Exosomal RNA extraction

Size Options

  • 25 Reactions
  • 50 Reactions

Species Reactivity

Pre-isolated exosomes from different human samples (biofluids or cell culture media, either pellet after precipitation, frozen, or lyophilized).

Applications

Exosome RNA extraction from pre-isolated exosomes pre-isolated via different methods including ultracentrifugation, chemical precipitation, immunocapture, size-chromatography etc, Simultaneous miRNA and mRNA profiling (qRT-PCR, RT-PCR, microarray).

Features and Benefits

High yield of total RNA (including small RNAs)

Kit Components

  • Lysis buffer
  • RNA Washing buffer (to add Ethanol 96%)
  • Elution buffer
  • Columns
  • Elution tubes
  • Lyophilized Exosome Standard

Storage Conditions

4°C

Shipping Conditions

Gel Pack

USAGE

For Research Use Only! Not For Use in Humans.

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