Anti-CAMK1D Antibody (CAB7512)

Antibody Name:	Anti-CAMK1D Antibody
Antibody SKU:	CAB7512
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human CAMK1D (NP_705718.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, 293T, Jurkat, Mouse thymus, Mouse spleen, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human CAMK1D (NP_705718.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MARE NGES SSSW KKQA EDIK KIFE FKET LGTG AFSE VVLA EEKA TGKL FAVK CIPK KALK GKES SIEN EIAV LRKI KHEN IVAL EDIY ESPN HLYL VMQL VSGG ELFD RIVE KGFY TEKD ASTL IRQV LDAV YYLH RMGI VHRD LKPE NLLY YSQD EESK IMIS DFGL SKME GKGD VMST
Gene ID:	57118
Uniprot:	Q8IU85
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	40kDa/42kDa
Observed MW:	45kDa

Synonyms:

CAMK1D, CKLiK, CaM-K1, CaMKID

Background:

This gene is a member of the calcium/calmodulin-dependent protein kinase 1 family, a subfamily of the serine/threonine kinases. The encoded protein is a component of the calcium-regulated calmodulin-dependent protein kinase cascade. It has been associated with multiple processes including regulation of granulocyte function, activation of CREB-dependent gene transcription, aldosterone synthesis, differentiation and activation of neutrophil cells, and apoptosis of erythroleukemia cells. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.

UniProt Protein Function:	CAMK1D: a calcium/calmodulin-dependent S/T protein kinase of the CAMK group. Binding of calmodulin is thought to result in a conformational change and can lead to subsequent activation through phosphorylation by CAMKK1 and CAMKK2. Can be activated by CAMKK1 in a calcium-independent manner. Its Ca(2+)/CaM-dependent activity is enhanced 30-fold in vitro by phosphorylation at Thr180 by CaMKK1. Broadly expressed. Highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. May regulate calcium-mediated granulocyte function. May play a role in apoptosis of erythroleukemia cells. In vitro, phosphorylates transcription factor CREM isoform Beta and probably CREB1. Expression increases upon treatment of EC cells with DMSO and retinoic acid. Two isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.17; CAMK group; CAMK1 family Chromosomal Location of Human Ortholog: 10p13 Cellular Component: cytoplasm; nucleus Molecular Function:calmodulin binding; calmodulin-dependent protein kinase activity; ATP binding Biological Process: positive regulation of apoptosis; positive regulation of phagocytosis; activation of CREB transcription factor; regulation of dendrite development; inflammatory response; protein amino acid phosphorylation; negative regulation of apoptosis
NCBI Summary:	This gene is a member of the calcium/calmodulin-dependent protein kinase 1 family, a subfamily of the serine/threonine kinases. The encoded protein is a component of the calcium-regulated calmodulin-dependent protein kinase cascade. It has been associated with multiple processes including regulation of granulocyte function, activation of CREB-dependent gene transcription, aldosterone synthesis, differentiation and activation of neutrophil cells, and apoptosis of erythroleukemia cells. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jan 2015]
UniProt Code:	Q8IU85
NCBI GenInfo Identifier:	56404610
NCBI Gene ID:	57118
NCBI Accession:	Q8IU85.1
UniProt Related Accession:	Q8IU85
Molecular Weight:	45kDa
NCBI Full Name:	Calcium/calmodulin-dependent protein kinase type 1D
NCBI Synonym Full Names:	calcium/calmodulin dependent protein kinase ID
NCBI Official Symbol:	CAMK1D
NCBI Official Synonym Symbols:	CKLiK; CaM-K1; CaMKID
NCBI Protein Information:	calcium/calmodulin-dependent protein kinase type 1D
UniProt Protein Name:	Calcium/calmodulin-dependent protein kinase type 1D
UniProt Synonym Protein Names:	CaM kinase I delta; CaM kinase ID; CaM-KI delta; CaMKI delta; CaMKID; CaMKI-like protein kinase; CKLiK
Protein Family:	Calcium/calmodulin-dependent protein kinase
UniProt Gene Name:	CAMK1D
UniProt Entry Name:	KCC1D_HUMAN

	Western blot analysis of extracts of various cell lines, using CAMK1D antibody (CAB7512) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
	Immunofluorescence analysis of A549 cells using CAMK1D antibody (CAB7512).

Antibodies

Anti-CAMK1D Antibody (CAB13435)

CAMK1D Antibody (PACO08153)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)