Metabolism Assays

About Acetaldehyde: ACETALDEHYDE (CH3CHO) is one of the most widely occurring aldehydes in nature and is commonly used in industry. Acetaldehyde, a metabolic byproduct of ethanol in the liver, is toxic to the human body and is rapidly converted to the...

About Acetaldehyde: ACETALDEHYDE (CH3CHO) is one of the most widely occurring aldehydes in nature and is commonly used in industry. Acetaldehyde, a metabolic byproduct of ethanol in the liver, is toxic to the human body and is rapidly converted to the...

Acetate Assay Kit Acetate is a common anion and fundamental to all forms of life. When bound to coenzyme A, it is central to the metabolism of carbohydrates and fats. It is acid form, acetic acid, is produced and excreted by acetic acid bacteria,...

Acetylcholine Assay Kit - InformationAssay Genie's acetylcholine assay provides a simple, direct and high-throughput assay for measuring acetylcholine in biological samples. In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline...

Acetylcholinesterase Assay Kit - InformationAssay Genie's colorimetic Acetylcholinesterase Assay Kit is based on an improved Ellman method, in which thiocholine produced by the action of acetylcholinesterase forms a yellow color with...

Acetylcholinesterase Inhibition Assay Kit ACETYLCHOLINESTERASE (EC 3.1.1.7, AChE), also known as RBC cholinesterase, is found primarily in the blood and neural synapses. AChE catalyzes the hydrolysis of the neurotransmitter acetylcholine into...

Acid Phosphatase Activity Assay Kit - InformationAssay Genie's non-radioactive, colorimetric acid phosphatase activity assay is based on the cleavage of p-nitrophenol from the synthetic substrate. p-Nitrophenol becomes intensely colored after addition of...

Acid Phosphatase Assay Kit Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes,...

Aconitase Assay Kit - InformationAssay Genie's aconitase assay measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense...

Adipolysis Assay Kit - InformationAssay Genie's adipolysis assay kit directly measures glycerol released during adipolysis. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions...

Alanine Transaminase Assay Kit - InformationAssay Genie's alanine transaminase activity assay (ALT) is based on the quantification of pyruvate produced by ALT. In this assay, pyruvate and NADH are converted to lactate and NAD by the enzyme lactate...

Alcohol dehydrogenase Activity Assay Kit - InformationAssay Genie's non-radioactive, colorimetric ADH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption...

Alkaline Phosphatase Assay - InformationAlkaline Phosphatase Assay Kit (Colorimetric) is designed to measure ALP activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP...

Alkaline Phosphatase Assay Kit - Information Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found...

ALP Detection Reagent: Assay Genie's ALP Detection Reagent is a stable 4-methylumbelliferyl phosphate (MUP) liquid substrate reagent formulated for detecting alkaline phosphatase ALP, e.g. in ELISA. This single reagent produces a highly fluorescent...

DescriptionAssay Genie's alpha-amylase assay method involves two steps: (1). alpha-amylase in the sample hydrolyzes starch and the product is rapidly converted to glucose by alpha-glucosidase and hydrogen peroxide by glucose oxidase; (2). hydrogen...

Alpha-Glucosidase Activity Assay Kit - InformationAlpha-Glucosidase colorimetric assay kit is designed to measure alpha-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes...

alpha-L- Fucosidase Assay Kit (AFU) - InformationAssay Genie' non-radioactive, colorimetric AFU assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of the stop reagent. The...

alpha-Mannosidase Activity Assay Kit - InformationAssay Genie's non-radioactive, colorimetric alpha-Mannosidase activity assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after addition of...

Ammonia Assay Kit Ammonia (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. In the liver, ammonia is...

Ammonia Assay Kit - InformationAssay Genie's ammonia assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ ammonium and forms a fluorescent product. The fluorescence intensity (Ex/Em = 360/450nm) is proportional to the...

Antioxidant Assay Kit - InformationAssay Genie's improved antioxidant assay measures total antioxidant capacity in which Cu2+ is reduced by antioxidant to Cu+. The resulting Cu+ specifically forms a colored complex with a dye reagent. The color intensity...

Arginase Activity Assay Kit - InformationAssay Genie's arginase assay kit provides a sensitive and convenient method for arginase activity determination. The method utilizes a chromogen that forms a colored complex specifically with urea produced in the...

About Arginase: ARGINASE (L-arginine ureohydrolase EC 3.5.3.1) is present in mammals and plants. In humans, arginase is expressed predominantly in the liver, and to lesser degrees in breast, kidney, testes, salivary glands, epidermis and erythrocytes...

Ascorbic Acid Assay Kit - InformationAssay Genie's ascorbic acid assay kit provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2...

Aspartate Assay Kit - InformationAssay Genie's Aspartate Assay Kit provides a simple, direct and automation-ready procedure for measuring aspartate concentration. Aspartate is converted into pyruvate which is then oxidized with the conversion of the dye...

Aspartate Transaminase Activity Assay Kit - InformationAssay Genie's Aspartate Transaminase (AST), also known as serum glutamic oxaloacetic transaminase (GOT) or aspartate aminotransferase (ASAT/AAT), facilitates the conversion of aspartate and...

BCG Albumin Assay Kit - InformationAssay Genie's BCG albumin assay kit is designed to measure albumin directly in biological samples without any pretreatment. The improved method utilizes bromcresol green that forms a colored complex specifically with...

BCP Albumin Assay Kit - InformationAssay Genie's BCP albumin assay kit is designed to measure albumin directly in biological samples without any pretreatment. The improved method utilizes bromcresol purple that forms a colored complex specifically with...

system_update_altDatasheetsystem_update_altMSDS Beta Hydroxybutyrate Assay Method of Detection: OD340nm Detection Limit: 0.12 mM Samples: Serum, plasma, urine etc Species: All Protocol Length: 30 min Size: 100 tests Shelf Life: 6...

beta-Glucosidase Activity Assay Kit - InformationAssay Genie's beta-Glucosidase Assay Kit is designed to measure beta-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes...

Beta-N-Acetylglucosaminidase Activity Assay Kit - Information Assay Genie' non-radioactive, colorimetric Beta-N-Acetylglucosaminidase Activity Assay Kit, NAG assay, is based on the cleavage of p-nitrophenol from a...

Bile Acid Assay Kit BILE ACIDS.Twelve different types of bile acids are typically found in mammals, among them two primary types are cholic acid and chenodeoxycholic acid. These can be dehydroxylated into secondary bile acids. Finally, these four can be...

Bilirubin Assay Kit - InformationAssay Genie's bilirubin assay kit is designed to measure bilirubin in blood specimen in 96-well or cuvette formats. The improved Jendrassik-Grof method utilizes the reaction of bilirubin with diazotized sulfanilic acid,...

Calcium Assay Kit - InformationAssay Genie's colorimetric calcium assay kit is designed to measure calcium directly in biological samples without any pretreatment. A phenolsulphonephthalein dye in the kit forms a very stable blue colored complex...

Catalase Activity Assay Kit Catalase Activity Assay Kit is a fast, easy and highly sensitive for measuring Catalase activity in biological samples.In the assay, catalase first reacts with H2O2 to producing water and...

Catalase Assay Kit - InformationAssay Genie's improved catalase assay directly measures catalase degradation of H2O2 using a redox dye. The change in color intensity at 570nm or fluorescence intensity (Ex/Em = 530/585nm) is directly proportional to the...

Chloride Assay Kit - InformationAssay Genie's chloride assay kit is designed to measure chloride directly in biological samples without any pretreatment. This Chloride Assay kit based on the improved Fried method utilizes mercuric...

Cholesterol Assay Kit - InformationAssay Genie's Cholesterol Assay (Colorimetric/Fluorometric) uses a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product...

Cholesterol Assay Kit - Information Assay Genie's colorimetric cholesterol assay is based on cholesterol esterase hydrolysis of cholesterol esters to form free cholesterol and cholesterol dehydrogenase catalyzed conversion of cholesterol to...

Cholesterol Uptake Assay Kit - Information Assay Genies total cholesterol assay kit (Fluorometric) is based on the cellular uptake of a fluorescently tagged cholesterol probe. The fluorescence intensity measured at Ex/Em = 485/535 nm is proportional...

system_update_altDatasheetsystem_update_altMSDS Choline Assay Kit - Information Assay Genie's choline assay provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by...

Chromium Release Assay - InformationAssay Genies Chromium Release Assay provides a simple one-step colorimetric means to directly measure chromium (Cr(VI)) in a sample. In the assay, chromium (Cr(VI)) forms a stable complex with a specific chromogenic...

Citrate Assay Kit - InformationAssay Genie's Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored...

Coenzyme A Assay Kit - InformationAssay Genie's Coenzyme A Assay Kit method provides a simple, two-step and high-throughput assay for measuring coenzyme A. In this assay, the first step enzymatically converts CoA to acyl-CoA and the second step oxidizes...

Copper Assay Kit - InformationAssay Genie's copper assay kit is designed to measure copper with no or minimal sample treatment. The improved method utilizes a chromogen that forms a colored complex specifically with copper ions. The intensity of the...

Creatine Assay Kit - InformationAssay Genie' creatine assay is based on enzymatic reactions leading to formation of a pink colored product. The optical density at 570 nm or fluorescence intensity at Ex/Em = 530/585 nm is directly proportional to the...

Creatine Kinase Activity Assay Kit - InformationAssay Genie's creatine kinase activity assay kit is based on enzyme coupled reactions in which creatine phosphate and ADP is converted to creatine and ATP by CK, the generated ATP is used to phosphorylate...

Creatinine Assay Kit - InformationAssay Genie's creatinine assay kit is designed to measure creatinine directly in biological samples without any pretreatment. The improved Jaffe method utilizes picrate that forms a red colored complex with creatinine...

D- Mannitol Assay D-MANNITOL (C6H14O6) is a sugar alcohol used in dietary supplement, sweetener, intestinal permeability test for leaky gut, etc. It also serves as a coating for hard candies, dried fruits, and chewing gums due to its low ability to...

D-Lactate Assay Kit - InformationAssay Genie's Colorimetric D-lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at...

D-Lactate Assay Kit Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is...

D-Sorbitol Assay Kit SORBITOL (glucitol) is a sugar alcohol that is metabolized slowly in the human body. Sorbitol can be obtained from glucoseby reducing aldehyde group to a hydroxyl group. Accumulation of excessive sorbitol in erythrocytes, retinal...

Diamine Oxidase Assay Kit DIAMINE OXIDASE (DAO) also known as histaminase or amine oxidase (copper containing), is an enzyme involved in the metabolism, oxidation, and inactivation of histamine in animals. Highest content is observed in the...

Ethanol Test Kit - InformationThis colorimetric ethanol test kit is based on an improved dichromate method, in which dichromate is reduced by ethanol to a bluish chromic (Cr3+) product. The intensity of color, measured at 580 nm, is a direct measure of...

Ethanol Assay Kit - InformationEthanol assay kit (colorimetric) is based on alcohol dehydrogenase catalyzed oxidation of ethanol, in which the formed NADH is coupled to the formazan (MTT) chromogen. The intensity of the product color, measured at 565 nm,...

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system_update_altDatasheet About Fatty Acids: Long Chain Fatty Acids (LCFA) are important fuel sources for animals as substrates in β-oxidation, and serve as building blocks for many different cellular structures. Long-chain unesterified fatty...

Formaldehyde Assay Kit - InformationAssay Genies newly designed formaldehyde assay kit provides a convenient fluorimetric means to measure formaldehyde in biological samples. In the assay, formaldehyde is derivatized with acetoacetanilide in the presence...

Formate Assay Kit FORMATE (HCOO-) is the anion derived from formic acid, the simplest carboxylic acid. It is also the metabolic byproduct of formaldehyde metabolism in our body, and the eventual metabolic byproduct of methanol which is first broken...

Free Fatty Acid Measurement Kit Fatty acidsare aliphatic monocarboxylic acids that are ubiquitously found in animal or vegetable fat, oil and wax. Fatty Acids play important roles in cellular synthesis, energy...

Fructose Assay Kit  Fructose (C6H12O6, also called levulose or laevulose), is a monosaccharide found in honey, tree fruits, berries, melons, and some root vegetables along with glucose and galactose. The human body can use fructose for energy,...

Fumarase Activity Assay Kit FUMARASE (or fumarate hydratase) (EC 4.2.1.2) is an enzyme that catalyzes the reversible hydration/dehydration reaction of fumarate to malate. Fumarase exists in two isoforms: a cytosolic and mitochondrial form. In the...

Fumarate Assay Kit FUMARATE , or Fumaric Acid, is one of the key components in TCA cycle and is used by cells to form ATP. Human skin when exposed to sunlight will naturally produce fumaric acid. Fumarate is used as an additive by the food and...

About Glucose-6-Phosphate Dehydrogenase: GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide...

Galactose Assay Kit  Galactose (C6H12O6) is a monosaccharide that is found in dairy products, sugar beets, gums and mucilages. It is also synthesized in mammals, where it forms part of glycolipids and glycoproteins in several tissues. It forms...

Glucose Assay Kit - InformatoionAssay Genie's glucose assay kit uses a single Working Reagent that combines the glucose oxidase reaction and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at...

Glucose Assay Kit - InformationAssay Genie' glucose assay kit is designed to measure glucose directly in serum or plasma without any pretreatment. The improved o-toluidine method utilizes a specific color reaction with glucose. The absorbance at 630nm is...

Glucose Dehydrogenase Assay Kit - InformationAssay Genie's non-radioactive, colorimetric GDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at...

Glucose Oxidase Assay Kit Glucose oxidase catalyzes the oxidation of glucose from D-glucose to D-glucono-lactone. Physiologically, it aids in the breakdown of glucose into smaller metabolites. It is widely used in electrochemical glucose sensors...

About Glucose: GLUCOSE (C6H12O6) is found in the food industry as either a natural component of the food or as an additive. Not only is glucose added to food and beverages for its impact on taste, texture, and color, but also because it can serve as a...

Glucose Uptake Assay Kit  Glucose Uptake has a variety of methods and transporters, and depends upon the metabolic demand of the cell type and availability of glucose. There are over ten different facilitated diffusion glucose transporters...

Glucose-6-Phosphate Assay Kit Glucose-6-Phosphate (g6p) is glucose sugar phosphorylated on carbon 6. Most of the glucose entering cells is phosphorylated to G6P. G6P has three primary fates within the cell. It lies at the start of two major...

Glucose 6 Phosphate Dehydrogenase Assay Kit - InformationAssay Genie's non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an...

Glutamate Assay Kit Glutamate is an important chemical in general metabolism. It is also a crucial mammalian neurotransmitter that is believed to be involved in a number of neurological and psychiatric disorders such as lateral sclerosis,...

Glutamate Dehyrogenase Activity Assay - InformationAssay Genie's non-radioactive, colorimetric GLDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption...

Glutamine Assay Kit - Information Glutamine is an amino acid synthesized in the muscle that plays major roles in protein synthesis, acid-base balance, anabolic processes and is utilized for cellular energy and as a carbon source. It is used in...

Glutathione Assay Kit - InformationAssay Genie's colorimetric Glutathione Assay Kit is designed to accurately measure reduced glutathione in biological samples. The improved 5,5'-dithiobis(2-nitrobenzoic acid (DTNB) method combines deproteination and...

Glutathione Peroxidase Assay Kit Glutathione peroxidase (GPX, EC 1.11.1.9) represents an enzyme family with peroxidase activity whose main biological role is to protect the organism from oxidative damage. It helps prevent lipid peroxidation of...

Glutathione Reductase Assay Kit - InformationAssay Genie's non-radioactive, colorimetric Glutathione Reductase Assay Kit is designed to accurately measure glutathione reductase (GR) activity in biological samples with a method that utilizes Ellman's...

Glycerol Assay Kit Glycerol [Glycerin or Glycerine, C3H5 (OH) 3] is widely used in foods, beverages and pharmaceutical formulations. It is also a main by-product of biodiesel production. Simple, direct and automation-ready procedures for...

Glycogen Assay Kit - InformationAssay Genie's glycogen assay uses a single Working Reagent that combines the enzymatic break down of glycogen and the detection of glucose in one step. The color intensity of the reaction product at 570nm or fluorescence...

Glycolysis Assay Kit - InformationAssay Genie's Glycolysis assay kit is based on measuring the production of L-Lactate from glycolysis in cells. L-Lactate that is secreted into the cell media when glycolysis occurs and is quantified using a coupled...

Glyoxalase I Activity Assay Kit - Information Assay Genie's Glyoxalase I assay kit provides a sensitive and convenient method for GLO-1 activity determination. The method involves monitoring the increase in the product of the GLO-1 reaction,...

GST Activity Assay Kit - InformationAssay Genie's Glutathione S-transferase, GST activity assay kit (colorimetric) is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1-chloro-2,4-dinitrobenzene). The GST catalyzed formation of...

HDL and LDL/VLDL Assay Kit - InformationHDL and LDL/VLDL Cholesterol Assay Kit quantification kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using a single...

HDL and LDL/VLDL Assay Kit Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing...

Heme Assay Kit - InformationAssay Genie's Heme Assay Kit is based on an improved aqueous alkaline solution method, in which the heme is converted into a uniform colored form. The intensity of color, measured at 400 nm, is directly proportional to the...

Hemoglobin Assay Kit - InformationAssay Genie's hemoglobin assay kit is based on an improved Triton/NaOH method, in which the hemoglobin is converted into a uniform colored end product. The intensity of color, measured at 400 nm, is directly proportional...

Histamine Assay Kit HISTAMINE (C5H9N3) is a biogenic amine naturally present in many foods and body cells in amounts without toxicological significance. It is also a contaminant that mostly found in the Scombridae family fishes such as tuna and...

About Histamine: HISTAMINE(C5H9N3) is a biogenic amine naturally present in many foods and body cells in amounts without toxicological significance. It is also a contaminant mostly found in Scombridae family fishes, such as tuna and mackerel, as well as...

HRP Detection Reagent: Assay Genie's fluorometric HRP detection reagent is a stable 10-Acetyl-3,7-dihydroxyphenoxazine (also called Ampliflu Red TM) liquid substrate reagent formulated for detecting horseradish peroxidase HRP activity, e.g. in ELISA...

Hydrogen Peroxide Assay Kit - InformationAssay Genie's hydrogen peroxide assay kit is designed to measure peroxide concentration in biological samples without any pretreatment. The improved method utilizes the chromogenic Fe3+-xylenol orange reaction, in...

Indican Assay Kit - InformationAssay Genie's quantitative indican assay kit is based on an improved Curzon and Walsh method. In this assay, indican reacts with a chromogen. The color intensity of the product at 480nm is directly proportional to the...

Indican Assay Kit - InformationAssay Genie's quantitative indican assay kit is based on an improved Curzon and Walsh method. In this assay, indican reacts with a chromogen. The color intensity of the product at 480nm is directly proportional to the...

Indole Assay Kit - Information Assay Genie's indole assay kit is based on a modified version of Ehlrich's and Kovac's reagents, which reacts with indole to produce a colored compound at 565 nm. The intensity of this colored compound is directly...

Invertase Activity Assay Kit Invertase (b-fructofuranosidase, EC 3.2.1.26) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertases cleave at the O-C(fructose) bond, whereas a related enzyme sucrase (EC 3.2.1.48)...

Iron Assay Kit - DescriptionAssay Genie's iron assay kit is designed to measure total iron directly in serum without any pretreatment. The improved method utilizes a chromogen that forms a blue colored complex specifically with Fe2+. Fe3+ in the sample...

Isocitrate Assay Kit - InformationAssay Genie's isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH converts the dye to an intense violet color with an absorption maximum at 565 nm. The increase in absorbance at 565...

Isocitrate Assay Kit ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing...