Multiplex ELISA
GeniePlex multiplex ELISA kits
Measure up to 24 cytokines and biomarkers from a single 15 µL sample. GeniePlex bead-based multiplex immunoassays run on any flow cytometer with a 488 nm laser, so there is no dedicated analyser to buy. Choose ready-made cytokine, inflammation, CAR-T and metabolic panels for human, mouse, rat and non-human primate samples, or build a custom panel.
Shop multiplex panels
Compare immunoassay formatsHow multiplex ELISA works, what you need and how to choose a panel
How bead-based multiplex ELISA works
Each analyte has its own antibody-coated bead population, identified by fluorescence. Beads capture their targets from the sample, a biotinylated detection antibody and streptavidin-PE add a reporter signal, and a flow cytometer reads bead identity and signal together to quantify every analyte in the same well.
What you need
Any flow cytometer with a 488 nm laser, a plate shaker and a way to wash beads, such as the GeniePlex filter plate washer. Panels are validated for serum, plasma, cell-culture supernatant, body fluids and tissue or cell lysates; each datasheet lists recovery, CV and cross-reactivity.
Multiplex or singleplex?
Choose multiplex when sample is scarce or you need a cytokine profile. Choose a singleplex ELISA kit when you need one analyte at the highest sensitivity or across many samples cheaply. For discovery screens of hundreds of proteins, see antibody arrays.
Custom panels and technology
Build a panel from our analyte menu with custom multiplex panels or the custom multiplex assay service. Learn more about GeniePlex technology and follow the step-by-step multiplex ELISA protocol.
There are no products listed under this category.