ADCC Assay Kit (BN00582)

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SKU:
BN00582
€433

Description

ELISA Kit Technical ManualMSDS

ADCC Assay Kit

Cell-mediated cytotoxicity is an important phenomenon characterized by cytolysis of a compromised cell in the body by immune system. Activation of immune system leads to removal of target cells infected by pathogens or transformed cells/cancer cells. This process is mediated by antibody-dependent cell-mediated cytotoxicity (ADCC), complement-mediated cytotoxicity, or lymphocyte-mediated cytotoxicity. Assay Genie's Cell-Mediated Cytotoxicity Assay Kit contains carboxyfluorosuccinimide ester (CFSE), a green fluorescent probe that labels live target cells and 7-aminoactinomycin D (7-AAD), a red fluorescent probe that labels late apoptotic and necrotic target cells killed in the cytotoxicity assay. This assay does not require cell lysis and provides a direct measurement of cytotoxicity instead of indirect indicators such as release of ATP or lactate dehydrogenase activity. The flow cytometry-based method provides robust data and enables multi-parametric analysis.

 
 
 

Figure: Cytotoxicity Assay Kit: Jurkat cells (105 cells/ml) were grown in RPMI media supplemented with 10% FBS. Cells were treated with camptothecin (5 µM) overnight. Next day, cells were stained with CFSE and 7-AAD for 30 min. at 37°C. The graph (right side) displays the cytotoxic effect of the compound, illustrating apoptosis using CFSE and 7-AAD.

Key Information Description

Product SKU

BN00582

Size

100 assays

Measurement

Fluorescence (FL1 channel and FL3 channel)

Kit Overview

Direct method to measure cell-mediated cytotoxicity. Does not require cell lysis. Provides multi-parametric analysis.
  • Measurement of cell cytotoxicity in response to drug or toxin treatment
  • Quantification of the cytotoxic effect of immune effector cells on target cells
  • Assessment of physiological mediators and antibodies that affect cell cytotoxicity

Kit Contents

  • Cytotoxicity Assay Buffer
  • 7-AAD Staining Solution
  • CFSE Staining Solution

Storage

-20°C

Shipping

Gel Pack

Research Use

For Research Use Only! Not For Use in Humans.

 
 
 
 
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