Anti-CYP2R1 Antibody (CAB10470)

SKU:
CAB10470
€99 - €249

Description

Technical Manual

Anti-CYP2R1 Antibody

Product Name:Anti-CYP2R1 Antibody
Product SKU:CAB10470
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. A mutation in this gene has been associated with selective 25-hydroxyvitamin D deficiency.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 252-501 of human CYP2R1 (NP_078790.2).
Sequence:

VVYDFLSRLIEKASVNRKPQLPQHFVDAYLDEMDQGKNDPSSTFSKENLIFSVGELIIAGTETTTNVLRWAILFMALYPNIQGQVQKEIDLIMGPNGKPSWDDKCKMPYTEAVLHEVLRFCNIVPLGIFHATSEDAVVRGYSIPKGTTVITNLYSVHFDEKYWRDPEVFHPERFLDSSGYFAKKEALVPFSLGRRHCLGEHLARMEMFLFFTALLQRFHLHFPHELVPDLKPRLGMTLQPQPYLICAERR

Gene ID:120227
Swiss Prot:Q6VVX0
Synonyms:CYP2R1
Calculated MW:57kDa
Observed MW:57kDa
Reactivity:Mouse, Rat
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting
Positive Samples:Mouse liver, Rat liver
Cellular Location:Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein

Product Images

Anti-CYP2R1 Antibody (CAB10470)

Western blot analysis of extracts of various cell lines, using CYP2R1 antibody (CAB10470) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

Uniprot Information

UniProt Protein Function:CYP2R1: Has a D-25-hydroxylase activity on both forms of vitamin D, vitamin D(2) and D(3). Defects in CYP2R1 are the cause of rickets vitamin D- dependent type 1B (VDDR1B); also known as pseudovitamin D(3) deficiency rickets due to 25-hydroxylase deficiency. A disorder caused by a selective deficiency of the active form of vitamin D (1,25-dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets. The patients sera have low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activityand low levels of 25-hydroxyvitamin D. Belongs to the cytochrome P450 family.
UniProt Protein Details:

Protein type:Oxidoreductase; EC 1.14.13.15

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function:iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; steroid hydroxylase activity; oxygen binding; vitamin D3 25-hydroxylase activity

Biological Process: steroid metabolic process; organic acid metabolic process; vitamin metabolic process; xenobiotic metabolic process; exogenous drug catabolic process; vitamin D metabolic process

Disease: Vitamin D Hydroxylation-deficient Rickets, Type 1b

NCBI Summary:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This enzyme is a microsomal vitamin D hydroxylase that converts vitamin D into the active ligand for the vitamin D receptor. A mutation in this gene has been associated with selective 25-hydroxyvitamin D deficiency. [provided by RefSeq, Jul 2008]
UniProt Code:Q6VVX0
NCBI GenInfo Identifier:62286619
NCBI Gene ID:120227
NCBI Accession:Q6VVX0.1
UniProt Secondary Accession:Q6VVX0,Q2M3H3, Q5RT65,
UniProt Related Accession:Q6VVX0
Molecular Weight:57,359 Da
NCBI Full Name:Vitamin D 25-hydroxylase
NCBI Synonym Full Names:cytochrome P450, family 2, subfamily R, polypeptide 1
NCBI Official Symbol:CYP2R1
NCBI Protein Information:vitamin D 25-hydroxylase; cytochrome P450 2R1; cytochrome P450, family 2, R1
UniProt Protein Name:Vitamin D 25-hydroxylase
UniProt Synonym Protein Names:Cytochrome P450 2R1
UniProt Gene Name:CYP2R1
UniProt Entry Name:CP2R1_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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