Anti-Cystatin-S Antibody (CAB8114)

Antibody Name:	CST4 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8114
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-141 of human CST4 (NP_001890.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-141 of human CST4 (NP_001890.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SSSK EENR IIPG GIYD ADLN DEWV QRAL HFAI SEYN KATE DEYY RRPL QVLR AREQ TFGG VNYF FDVE VGRT ICTK SQPN LDTC AFHE QPEL QKKQ LCSF EIYE VPWE DRMS LVNS RCQE A
Gene ID:	1472
Uniprot:	P01036
Cellular Location:	Secreted
Calculated MW:	16kDa
Observed MW:	16kDa

Synonyms:

CST4

Background:

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a type 2 salivary cysteine peptidase inhibitor. The protein is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function.

UniProt Protein Function:	CST4: This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively. Belongs to the cystatin family.
UniProt Protein Details:	Protein type:Inhibitor; Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 20p11.21 Cellular Component: extracellular space Molecular Function:cysteine protease inhibitor activity; protease binding Biological Process: detection of chemical stimulus involved in sensory perception of bitter taste; negative regulation of proteolysis; retinal homeostasis
NCBI Summary:	The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a type 2 salivary cysteine peptidase inhibitor. The protein is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function. [provided by RefSeq, Jul 2008]
UniProt Code:	P01036
NCBI GenInfo Identifier:	4503109
NCBI Gene ID:	1472
NCBI Accession:
UniProt Secondary Accession:	P01036,Q9UBI5, Q9UCS9,
UniProt Related Accession:	P01036
Molecular Weight:	16,214 Da
NCBI Full Name:	cystatin-S
NCBI Synonym Full Names:	cystatin S
NCBI Official Symbol:	CST4
NCBI Protein Information:	cystatin-S
UniProt Protein Name:	Cystatin-S
UniProt Synonym Protein Names:	Cystatin-4; Cystatin-SA-III; Salivary acidic protein 1
Protein Family:	Cystatin
UniProt Gene Name:	CST4
UniProt Entry Name:	CYTS_HUMAN

	Western blot analysis of extracts of BT-474 cells, using CST4 antibody (CAB8114) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded rat lung using CST4 antibody (CAB8114) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using CST4 antibody (CAB8114) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)