Anti-DDX46 Antibody (CAB4350)

Antibody Name:	Anti-DDX46 Antibody
Antibody SKU:	CAB4350
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 682-1031 of human DDX46 (NP_055644.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 682-1031 of human DDX46 (NP_055644.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GLDV KHLI LVVN YSCP NHYE DYVH RAGR TGRA GNKG YAYT FITE DQAR YAGD IIKA LELS GTAV PPDL EKLW SDFK DQQK AEGK IIKK SSGF SGKG FKFD ETEQ ALAN ERKK LQKA ALGL QDSD DEDA AVDI DEQI ESMF NSKK RVKD MAAP GTSS VPAP TAGN AEKL EIAK RLAL RINA QKNL GIES QDVM QQAT NAIL RGGT ILAP TVSA KTIA EQLA EKIN AKLN YVPL EKQE EERQ DGGQ NESF KRYE EELE INDF PQTA RWKV TSKE ALQR ISEY SEAA ITIR GTYF PPGK EPKE GERK IYLA IESA NELA VQKA KAEI TRLI KEEL IRLQ NSYQ PTNK GRYK VL
Gene ID:	9879
Uniprot:	Q7L014
Cellular Location:	Cajal body, Lipid-anchor, Membrane, Nucleus, Nucleus speckle
Calculated MW:	117kDa
Observed MW:	117kDa

Synonyms:

PRPF5, Prp5, DDX46

Background:

This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing. Multiple alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	DDX46: Plays an essential role in splicing, either prior to, or during splicing A complex formation. Belongs to the DEAD box helicase family. DDX46/PRP5 subfamily.
UniProt Protein Details:	Protein type:Spliceosome; Helicase; EC 3.6.4.13 Chromosomal Location of Human Ortholog: 5q31.1 Cellular Component: Cajal body; nuclear speck; nucleus Molecular Function:helicase activity; ATP binding Biological Process: RNA splicing; mRNA processing
NCBI Summary:	This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014]
UniProt Code:	Q7L014
NCBI GenInfo Identifier:	116241326
NCBI Gene ID:	9879
NCBI Accession:	Q7L014.2
UniProt Related Accession:	Q7L014
Molecular Weight:	~ 117kDa
NCBI Full Name:	Probable ATP-dependent RNA helicase DDX46
NCBI Synonym Full Names:	DEAD-box helicase 46
NCBI Official Symbol:	DDX46
NCBI Official Synonym Symbols:	Prp5; PRPF5
NCBI Protein Information:	probable ATP-dependent RNA helicase DDX46
UniProt Protein Name:	Probable ATP-dependent RNA helicase DDX46
UniProt Synonym Protein Names:	DEAD box protein 46; PRP5 homolog
Protein Family:	Probable ATP-dependent RNA helicase
UniProt Gene Name:	DDX46
UniProt Entry Name:	DDX46_HUMAN

	Western blot analysis of extracts of various cell lines, using DDX46 antibody (CAB4350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded rat testis using DDX46 antibody (CAB4350) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using DDX46 antibody (CAB4350) at dilution of 1:100 (40x lens).

Antibodies

Anti-DDX46 Antibody (CAB18494)

DDX46 Antibody (PACO08794)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)