Anti-DNASE1L1 Antibody (CAB3831)

Antibody Name:	Anti-DNASE1L1 Antibody
Antibody SKU:	CAB3831
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human DNASE1L1 (NP_006721.1).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human DNASE1L1 (NP_006721.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MHYP TALL FLIL ANGA QAFR ICAF NAQR LTLA KVAR EQVM DTLV RILA RCDI MVLQ EVVD SSGS AIPL LLRE LNRF DGSG PYST LSSP QLGR STYM ETYV YFYR SHKT QVLS SYVY NDED DVFA REPF VAQF SLPS NVLP SLVL VPLH TTPK AVEK ELNA LYDV FLEV SQHW QSKD VILL GDFN ADCA SLTK KRLD KLEL RTEP GFHW VIAD GEDT TVRA STHC TYDR VVLH GERC RSLL HTAA AFDF PTSF QLTE EEAL NISD HYPV EVEL KLSQ AHSV QPLS LTVL LLLS LLSP QLCP AA
Gene ID:	1774
Uniprot:	P49184
Cellular Location:	Endoplasmic reticulum
Calculated MW:	33kDa
Observed MW:	36kDa

Synonyms:	DNASE1L1, DNAS1L1, DNASEX, DNL1L, G4.8, XIB
Background:	This gene encodes a deoxyribonuclease protein that shows high sequence similarity to DNase I. The encoded protein is localized to the endoplasmic reticulum and modified by N-linked glycosylation. Alternate transcriptional splice variants encoding the same protein have been observed.

UniProt Protein Function:	DNASE1L1: a member of the deoxyribonuclease family and the protein shows high sequence similarity to lysosomal DNase I. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:EC 3.1.21.-; Deoxyribonuclease Chromosomal Location of Human Ortholog: Xq28 Cellular Component: endoplasmic reticulum; nucleus Molecular Function:endodeoxyribonuclease activity, producing 5'-phosphomonoesters; DNA binding; deoxyribonuclease activity; endodeoxyribonuclease activity Biological Process: DNA catabolic process, endonucleolytic; DNA metabolic process
NCBI Summary:	This gene encodes a member of the deoxyribonuclease family and the protein shows high sequence similarity to lysosomal DNase I. Alternate transcriptional splice variants, encoding the same protein, have been characterized. [provided by RefSeq, Jul 2008]
UniProt Code:	P49184
NCBI GenInfo Identifier:	1352319
NCBI Gene ID:	1774
NCBI Accession:	P49184.1
UniProt Secondary Accession:	P49184,Q5HY41, D3DWW7,
UniProt Related Accession:	P49184
Molecular Weight:	33,893 Da
NCBI Full Name:	Deoxyribonuclease-1-like 1
NCBI Synonym Full Names:	deoxyribonuclease I-like 1
NCBI Official Symbol:	DNASE1L1
NCBI Official Synonym Symbols:	XIB; G4.8; DNL1L; DNASEX; DNAS1L1
NCBI Protein Information:	deoxyribonuclease-1-like 1; DNase X; DNase I-like 1; DNase I-like, muscle-specific
UniProt Protein Name:	Deoxyribonuclease-1-like 1
UniProt Synonym Protein Names:	DNase X; Deoxyribonuclease I-like 1; DNase I-like 1; Muscle-specific DNase I-like; XIB
UniProt Gene Name:	DNASE1L1
UniProt Entry Name:	DNSL1_HUMAN

Western blot analysis of extracts of MCF7 cells, using DNASE1L1 Antibody (CAB3831) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Antibodies

Anti-DNASE1L1 Antibody (CAB13150)

DNASE1L1 Antibody (PACO08898)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)