Anti-DPM1 Antibody (CAB7757)

Antibody Name:	Anti-DPM1 Antibody
Antibody SKU:	CAB7757
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DPM1 (NP_003850.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	NCI-H460, HeLa, SKOV3, Mouse pancreas, Mouse liver, Mouse kidney, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DPM1 (NP_003850.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASL EVSR SPRR SRRE LEVR SPRQ NKYS VLLP TYNE RENL PLIV WLLV KSFS ESGI NYEI IIID DGSP DGTR DVAE QLEK IYGS DRIL LRPR EKKL GLGT AYIH GMKH ATGN YIII MDAD LSHH PKFI PEFI RKQK EGNF DIVS GTRY KGNG GVYG WDLK RKII SRGA NFLT QILL RPGA SDLT GSFR LYRK EVLE KLIE KCVS KGYV FQME MIVR ARQL NYTI GEVP ISFV DRVY GESK LGGN EIVS FLKG LLTL FATT
Gene ID:	8813
Uniprot:	O60762
Cellular Location:	Endoplasmic reticulum
Calculated MW:	29kDa
Observed MW:	30kDa

Synonyms:

DPM1, CDGIE, MPDS

Background:

Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. Mutations in this gene are associated with congenital disorder of glycosylation type Ie. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	DPM1: Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O- mannosylation of proteins. Defects in DPM1 are the cause of congenital disorder of glycosylation type 1E (CDG1E). CDGs are metabolic deficiencies in glycoprotein biosynthesis that usually cause severe mental and psychomotor retardation. They are characterized by under-glycosylated serum glycoproteins. CDG1E is an autosomal recessive disorder, characterized by severe developmental delay, hypotnia, seizures, and dysmorphic features. Belongs to the glycosyltransferase 2 family.
UniProt Protein Details:	Protein type:Glycan Metabolism - N-glycan biosynthesis; EC 2.4.1.83; Transferase Chromosomal Location of Human Ortholog: 20q13.13 Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum; nucleus Molecular Function:mannose binding; protein binding; alcohol binding; dolichyl-phosphate-mannose-protein mannosyltransferase activity; dolichyl-phosphate beta-D-mannosyltransferase activity Biological Process: cellular protein metabolic process; protein amino acid mannosylation; protein amino acid O-linked mannosylation; dolichol-linked oligosaccharide biosynthetic process; GDP-mannose metabolic process; GPI anchor biosynthetic process; C-terminal protein lipidation; dolichol metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification Disease: Congenital Disorder Of Glycosylation, Type Ie
NCBI Summary:	Dolichol-phosphate mannose (Dol-P-Man) serves as a donor of mannosyl residues on the lumenal side of the endoplasmic reticulum (ER). Lack of Dol-P-Man results in defective surface expression of GPI-anchored proteins. Dol-P-Man is synthesized from GDP-mannose and dolichol-phosphate on the cytosolic side of the ER by the enzyme dolichyl-phosphate mannosyltransferase. Human DPM1 lacks a carboxy-terminal transmembrane domain and signal sequence and is regulated by DPM2. [provided by RefSeq, Jul 2008]
UniProt Code:	O60762
NCBI GenInfo Identifier:	48145969
NCBI Gene ID:	8813
NCBI Accession:	CAG33207.1
UniProt Secondary Accession:	O60762,O15157, Q6IB78, Q96HK0,
UniProt Related Accession:	O60762
Molecular Weight:	29,634 Da
NCBI Full Name:	DPM1
NCBI Synonym Full Names:	dolichyl-phosphate mannosyltransferase polypeptide 1, catalytic subunit
NCBI Official Symbol:	DPM1
NCBI Official Synonym Symbols:	MPDS; CDGIE
NCBI Protein Information:	dolichol-phosphate mannosyltransferase subunit 1; DPM synthase complex, catalytic subunit; DPM synthase subunit 1; MPD synthase subunit 1; dolichol monophosphate mannose synthase; dolichol-phosphate mannose synthase subunit 1; dolichyl-phosphate beta-D-mannosyltransferase subunit 1; mannose-P-dolichol synthase subunit 1
UniProt Protein Name:	Dolichol-phosphate mannosyltransferase subunit 1
UniProt Synonym Protein Names:	Dolichol-phosphate mannose synthase subunit 1; DPM synthase subunit 1; Dolichyl-phosphate beta-D-mannosyltransferase subunit 1; Mannose-P-dolichol synthase subunit 1; MPD synthase subunit 1
Protein Family:	Dolichol-phosphate mannosyltransferase
UniProt Gene Name:	DPM1
UniProt Entry Name:	DPM1_HUMAN

	Western blot analysis of extracts of various cell lines, using DPM1 antibody (CAB7757) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using DPM1 antibody (CAB7757) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

DPM1 Antibody (PACO08922)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)