Anti-Dual oxidase 1 Antibody (CAB8583)

Antibody Name:	Anti-Dual oxidase 1 Antibody
Antibody SKU:	CAB8583
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1312-1551 of human DUOX1 (NP_059130.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Mouse, Rat
Positive Samples:	Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1312-1551 of human DUOX1 (NP_059130.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ALGT TEYH PFTL TSAP HEDT LSLH IRAA GPWT TRLR EIYS APTG DRCA RYPK LYLD GPFG EGHQ EWHK FEVS VLVG GGIG VTPF ASIL KDLV FKSS VSCQ VFCK KIYF IWVT RTQR QFEW LADI IREV EEND HQDL VSVH IYIT QLAE KFDL RTTM LYIC ERHF QKVL NRSL FTGL RSIT HFGR PPFE PFFN SLQE VHPQ VRKI GVFS CGPP GMTK NVEK ACQL INRQ DRTH FSHH YENF
Gene ID:	53905
Uniprot:	Q9NRD9
Cellular Location:	Apical cell membrane, Multi-pass membrane protein
Calculated MW:	137kDa/177kDa
Observed MW:	180kDa

Synonyms:

DUOX1, LNOX1, NOXEF1, THOX1

Background:

The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes proteins encoded by this gene and the similar DUOX2 gene. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. This protein generates hydrogen peroxide and thereby plays a role in the activity of thyroid peroxidase, lactoperoxidase, and in lactoperoxidase-mediated antimicrobial defense at mucosal surfaces. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.

UniProt Protein Function:	DUOX1: Generates hydrogen peroxide which is required for the activity of thyroid peroxidase/TPO and lactoperoxidase/LPO. Plays a role in thyroid hormones synthesis and lactoperoxidase-mediated antimicrobial defense at the surface of mucosa. May have its own peroxidase activity through its N-terminal peroxidase-like domain. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral; EC 1.6.3.1; Oxidoreductase Chromosomal Location of Human Ortholog: 15q15.3 Cellular Component: apical plasma membrane; integral to membrane; plasma membrane Molecular Function:calcium ion binding; heme binding; NAD(P)H oxidase activity; NADP binding; peroxidase activity Biological Process: cuticle development; cytokine and chemokine mediated signaling pathway; hormone biosynthetic process; hydrogen peroxide biosynthetic process; hydrogen peroxide catabolic process; response to cAMP; response to oxidative stress; superoxide release; thyroid hormone generation
NCBI Summary:	The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes proteins encoded by this gene and the similar DUOX2 gene. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. This protein generates hydrogen peroxide and thereby plays a role in the activity of thyroid peroxidase, lactoperoxidase, and in lactoperoxidase-mediated antimicrobial defense at mucosal surfaces. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2012]
UniProt Code:	Q9NRD9
NCBI GenInfo Identifier:	20149640
NCBI Gene ID:	53905
NCBI Accession:	NP_059130.2
UniProt Secondary Accession:	Q9NRD9,Q14C94, Q6ZMB3, Q6ZR09, Q9NZC1, A6NH28,
UniProt Related Accession:	Q9NRD9
Molecular Weight:	Calculated: 137kDa/177kDa; Observed: 180 kDa
NCBI Full Name:	dual oxidase 1
NCBI Synonym Full Names:	dual oxidase 1
NCBI Official Symbol:	DUOX1
NCBI Official Synonym Symbols:	LNOX1; THOX1; NOXEF1
NCBI Protein Information:	dual oxidase 1
UniProt Protein Name:	Dual oxidase 1
UniProt Synonym Protein Names:	Large NOX 1; Long NOX 1; NADPH thyroid oxidase 1; Thyroid oxidase 1
Protein Family:	Dual oxidase
UniProt Gene Name:	DUOX1
UniProt Entry Name:	DUOX1_HUMAN

Anti-Dual oxidase 1 Polyclonal Antibody (CAB8583)

Western blot analysis of extracts of various cell lines, using DUOX1 antibody (CAB8583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)