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Anti-GMDS Antibody (CAB15060)

SKU:
CAB15060
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€139 - €419
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Description

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Antibody Name:Anti-GMDS Antibody
Antibody SKU:CAB15060
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GMDS (NP_001491.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:HeLa
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GMDS (NP_001491.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAHA PARC PSAR GSGD GEMG KPRN VALI TGIT GQDG SYLA EFLL EKGY EVHG IVRR SSSF NTGR IEHL YKNP QAHI EGNM KLHY GDLT DSTC LVKI INEV
Gene ID:2762
Uniprot:O60547
Cellular Location:
Calculated MW:39kDa/41kDa
Observed MW:42kDa
Synonyms:GMDS, GMD, SDR3E1, GDP-mannose 4
Background:
UniProt Protein Function:GMD: an enzyme that converts GDP-D-mannose to GDP-4-keto-6-D- deoxymannose. Perticipates in the extravasation of activated lymphocytes.
UniProt Protein Details:

Protein type:Lyase; Carbohydrate Metabolism - fructose and mannose; EC 4.2.1.47; Carbohydrate Metabolism - amino sugar and nucleotide sugar

Chromosomal Location of Human Ortholog: 6p25

Cellular Component: cytoplasm

Molecular Function:GDP-mannose 4,6-dehydratase activity

Biological Process: Notch signaling pathway; GDP-mannose metabolic process; 'de novo' GDP-L-fucose biosynthetic process

NCBI Summary:GDP-mannose 4,6-dehydratase (GMD; EC 4.2.1.47) catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor. The second and third steps of the pathway are catalyzed by a single enzyme, GDP-keto-6-deoxymannose 3,5-epimerase, 4-reductase, designated FX in humans (MIM 137020).[supplied by OMIM, Aug 2009]
UniProt Code:O60547
NCBI GenInfo Identifier:9087147
NCBI Gene ID:2762
NCBI Accession:O60547.1
UniProt Secondary Accession:O60547,O75357, Q5T954, Q6FH09, Q9UGZ3, Q9UJK9, E9PI88
UniProt Related Accession:O60547
Molecular Weight:372
NCBI Full Name:GDP-mannose 4,6 dehydratase
NCBI Synonym Full Names:GDP-mannose 4,6-dehydratase
NCBI Official Symbol:GMDS  
NCBI Official Synonym Symbols:GMD; SDR3E1  
NCBI Protein Information:GDP-mannose 4,6 dehydratase; GDP-D-mannose dehydratase; short chain dehydrogenase/reductase family 3E, member 1
UniProt Protein Name:GDP-mannose 4,6 dehydratase
UniProt Synonym Protein Names:GDP-D-mannose dehydratase; GMD
Protein Family:GDP-mannose 4,6 dehydratase
UniProt Gene Name:GMDS  
UniProt Entry Name:GMDS_HUMAN
Anti-GMDS Antibody (CAB15060)Western blot analysis of extracts of HeLa cells, using GMDS antibody (CAB15060) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60.

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