Anti-GPR143 Antibody (CAB10568)

Antibody Name:	Anti-GPR143 Antibody
Antibody SKU:	CAB10568
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 314-404 of human GPR143 (NP_000264.2).

Application:	WB
Recommended Dilution:	WB 1:1000 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, BT-474, SW480, Mouse liver, Mouse kidney, Rat kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 314-404 of human GPR143 (NP_000264.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TGCS LGFQ SPRK EIQW ESLT TSAA EGAH PSPL MPHE NPAS GKVS QVGG QTSD EALS MLSE GSDA STIE IHTA SESC NKNE GDPA LPTH GDL
Gene ID:	4935
Uniprot:	P51810
Cellular Location:	Apical cell membrane, Golgi apparatus, Lysosome membrane, Melanosome membrane, Multi-pass membrane protein
Calculated MW:	43kDa
Observed MW:	44kDa

Synonyms:

GPR143, NYS6, OA1

Background:

This gene encodes a protein that binds to heterotrimeric G proteins and is targeted to melanosomes in pigment cells. This protein is thought to be involved in intracellular signal transduction mechanisms. Mutations in this gene cause ocular albinism type 1, also referred to as Nettleship-Falls type ocular albinism, a severe visual disorder. A related pseudogene has been identified on chromosome Y.

UniProt Protein Function:	GPR143: Receptor for tyrosine, L-DOPA and dopamine. After binding to L-DOPA, stimulates Ca(2+) influx into the cytoplasm, increases secretion of the neurotrophic factor SERPINF1 and relocalizes beta arrestin at the plasma membrane; this ligand- dependent signaling occurs through a G(q)-mediated pathway in melanocytic cells. Its activity is mediated by G proteins which activate the phosphoinositide signaling pathway. Plays also a role as an intracellular G protein-coupled receptor involved in melanosome biogenesis, organization and transport. Defects in GPR143 are the cause of albinism ocular type 1 (OA1); also known as Nettleship-Falls type ocular albinism. Form of albinism affecting only the eye. Pigment of the hair and skin is normal or only slightly diluted. Eyes may be severely affected with photophobia and reduced visual acuity. Nystagmus or strabismus are often associated. The irides and fundus are depigmented. Defects in GPR143 are the cause of Nystagmus congenital X-linked type 6 (NYS6). It is a condition defined as conjugated, spontaneous and involuntary ocular oscillations that appear at birth or during the first three months of life. Other associated features may include mildly decreased visual acuity, strabismus, astigmatism, and occasionally head nodding. Belongs to the G-protein coupled receptor OA family.
UniProt Protein Details:	Protein type:GPCR, OA family; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR Chromosomal Location of Human Ortholog: Xp22.2 Cellular Component: apical plasma membrane; cytoplasm; Golgi apparatus; lysosomal membrane; melanosome; melanosome membrane; membrane; plasma membrane Molecular Function:dopamine binding; G-protein coupled receptor activity; protein binding Biological Process: eye pigment biosynthetic process; G-protein coupled receptor protein signaling pathway; melanosome localization; melanosome organization and biogenesis; melanosome transport; phosphoinositide-mediated signaling; regulation of calcium-mediated signaling; signal transduction; visual perception Disease: Albinism, Ocular, Type I; Nystagmus 6, Congenital, X-linked
NCBI Summary:	This gene encodes a protein that binds to heterotrimeric G proteins and is targeted to melanosomes in pigment cells. This protein is thought to be involved in intracellular signal transduction mechanisms. Mutations in this gene cause ocular albinism type 1, also referred to as Nettleship-Falls type ocular albinism, a severe visual disorder. A related pseudogene has been identified on chromosome Y. [provided by RefSeq, Dec 2009]
UniProt Code:	P51810
NCBI GenInfo Identifier:	3219999
NCBI Gene ID:	4935
NCBI Accession:	P51810.2
UniProt Secondary Accession:	P51810,Q6NTI7,
UniProt Related Accession:	P51810
Molecular Weight:	44kDa
NCBI Full Name:	G-protein coupled receptor 143
NCBI Synonym Full Names:	G protein-coupled receptor 143
NCBI Official Symbol:	GPR143
NCBI Official Synonym Symbols:	OA1; NYS6
NCBI Protein Information:	G-protein coupled receptor 143
UniProt Protein Name:	G-protein coupled receptor 143
UniProt Synonym Protein Names:	Ocular albinism type 1 protein
Protein Family:	G-protein coupled receptor
UniProt Gene Name:	GPR143

Western blot analysis of extracts of various cell lines, using GPR143 antibody (CAB10568) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.

Antibodies

GPR143 Antibody (PACO04476)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)