Anti-HEBP2 Antibody (CAB16504)

Antibody Name:	Anti-HEBP2 Antibody
Antibody SKU:	CAB16504
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human HEBP2 (NP_055135.1).

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human HEBP2 (NP_055135.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAEP LQPD PGAA EDAA AQAV ETPG WKAP EDAG PQPG SYEI RHYG PAKW VSTS VESM DWDS AIQT GFTK LNSY IQGK NEKE MKIK MTAP VTSY VEPG SGPF SEST ITIS LYIP SEQQ FDPP RPLE SDVF IEDR AEMT VFVR SFDG FSSA QKNQ EQLL TLAS ILRE DGKV FDEK VYYT AGYN SPVK LLNR NNEV WLIQ KNEP TKEN E
Gene ID:	23593
Uniprot:	Q9Y5Z4
Cellular Location:	Cytoplasm, Mitochondrion
Calculated MW:	20kDa/22kDa
Observed MW:	25kDa

Synonyms:	HEBP2, C6ORF34B, C6orf34, PP23, SOUL
Background:	The protein encoded by this gene is found predominately in the cytoplasm, where it plays a role in the collapse of mitochondrial membrane potential (MMP) prior to necrotic cell death. The encoded protein enhances outer and inner mitochondrial membrane permeabilization, especially under conditions of oxidative stress.

UniProt Protein Function:	HEBP2: Can promote mitochondrial permeability transition and facilitate necrotic cell death under different types of stress conditions. Does not bind hemin. Belongs to the HEBP family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 6q24 Cellular Component: cytoplasm; mitochondrion Molecular Function:protein binding
NCBI Summary:	The protein encoded by this gene is found predominately in the cytoplasm, where it plays a role in the collapse of mitochondrial membrane potential (MMP) prior to necrotic cell death. The encoded protein enhances outer and inner mitochondrial membrane permeabilization, especially under conditions of oxidative stress. [provided by RefSeq, May 2016]
UniProt Code:	Q9Y5Z4
NCBI GenInfo Identifier:	74753513
NCBI Gene ID:	23593
NCBI Accession:	Q9Y5Z4.1
UniProt Secondary Accession:	Q9Y5Z4,Q96P57,
UniProt Related Accession:	Q9Y5Z4
Molecular Weight:	20,859 Da
NCBI Full Name:	Heme-binding protein 2
NCBI Synonym Full Names:	heme binding protein 2
NCBI Official Symbol:	HEBP2
NCBI Official Synonym Symbols:	PP23; SOUL; C6orf34; C6ORF34B
NCBI Protein Information:	heme-binding protein 2
UniProt Protein Name:	Heme-binding protein 2
UniProt Synonym Protein Names:	Placental protein 23; PP23; Protein SOUL
Protein Family:	Heme-binding protein
UniProt Gene Name:	HEBP2
UniProt Entry Name:	HEBP2_HUMAN

	Western blot analysis of extracts of various cell lines, using HEBP2 antibody (CAB16504) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded Rat spleen using HEBP2 Rabbit pAb (CAB16504) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Mouse kidney using HEBP2 Rabbit pAb (CAB16504) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using HEBP2 antibody (CAB16504) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using HEBP2 antibody (CAB16504) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Secondary Antibody