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Anti-HS2ST1 Antibody (CAB13837)

SKU:
CAB13837
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

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Antibody Name:Anti-HS2ST1 Antibody
Antibody SKU:CAB13837
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-229 of human HS2ST1 (NP_001127964.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, Mouse lung, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-229 of human HS2ST1 (NP_001127964.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGLL RIMM PPKL QLLA VVAF AVAM LFLE NQIQ KLEE SRSK LERA IARH EVRE IEQR HTMD GPRQ DATL DEEE DMVI IYNR VPKT ASTS FTNI AYDL CAKN KYHV LHIN TTKN NPVM SLQD QVRF VKNI TSWK EMKP GFYH GHVS YLDF AKFG VKKK PIYI NVIR DPIE RLVS YYYF LRFG DDYR PGLR RRKQ GDKK TFDE CVAE GGSD CAPE KLWL QIPF FCGH SSEC W
Gene ID:9653
Uniprot:Q7LGA3
Cellular Location:Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:26kDa/34kDa/41kDa
Observed MW:42kDa
Synonyms:HS2ST1, dJ604K5.2
Background:Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. This gene encodes a member of the heparan sulfate biosynthetic enzyme family that transfers sulfate to the 2 position of the iduronic acid residue of heparan sulfate. The disruption of this gene resulted in no kidney formation in knockout embryonic mice, indicating that the absence of this enzyme may interfere with the signaling required for kidney formation. Two alternatively spliced transcript variants that encode different proteins have been found for this gene.
UniProt Protein Function:HS2ST1: Catalyzes the transfer of sulfate to the C2-position of selected hexuronic acid residues within the maturing heparan sulfate (HS). 2-O-sulfation within HS, particularly of iduronate residues, is essential for HS to participate in a variety of high- affinity ligand-binding interactions and signaling processes. Mediates 2-O-sulfation of both L-iduronyl and D-glucuronyl residues. Belongs to the sulfotransferase 3 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Membrane protein, integral; Glycan Metabolism - heparan sulfate biosynthesis; EC 2.8.2.-; Transferase

Chromosomal Location of Human Ortholog: 1p22.3

Cellular Component: Golgi membrane; membrane

Molecular Function:heparan sulfate 2-O-sulfotransferase activity

Biological Process: glycosaminoglycan biosynthetic process

NCBI Summary:Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. This gene encodes a member of the heparan sulfate biosynthetic enzyme family that transfers sulfate to the 2 position of the iduronic acid residue of heparan sulfate. The disruption of this gene resulted in no kidney formation in knockout embryonic mice, indicating that the absence of this enzyme may interfere with the signaling required for kidney formation. Two alternatively spliced transcript variants that encode different proteins have been found for this gene. [provided by RefSeq, Aug 2008]
UniProt Code:Q7LGA3
NCBI GenInfo Identifier:68052326
NCBI Gene ID:9653
NCBI Accession:Q7LGA3.1
UniProt Secondary Accession:Q7LGA3,O75036, Q32NB5, Q8TAC5, Q9H441, Q9NUJ9, D3DT22
UniProt Related Accession:Q7LGA3
Molecular Weight:26,757 Da
NCBI Full Name:Heparan sulfate 2-O-sulfotransferase 1
NCBI Synonym Full Names:heparan sulfate 2-O-sulfotransferase 1
NCBI Official Symbol:HS2ST1  
NCBI Official Synonym Symbols:dJ604K5.2  
NCBI Protein Information:heparan sulfate 2-O-sulfotransferase 1
UniProt Protein Name:Heparan sulfate 2-O-sulfotransferase 1
Protein Family:Heparan sulfate 2-O-sulfotransferase
UniProt Gene Name:HS2ST1  
UniProt Entry Name:HS2ST_HUMAN
Anti-HS2ST1 Antibody (CAB13837)Western blot analysis of extracts of various cell lines, using HS2ST1 antibody (CAB13837) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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