Anti-IL-12RB2 Antibody (CAB10348)

SKU:
CAB10348
€99 - €249

Description

Technical Manual

IL12RB2 Rabbit Polyclonal Antibody

Product Name:IL12RB2 Rabbit Polyclonal Antibody
Product SKU:CAB10348
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. Several transcript variants encoding different isoforms and non-protein coding transcripts have been found for this gene.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 420-620 of human IL12RB2 (NP_001550.1).
Sequence:

GLLAPRQVSANSEGMDNILVTWQPPRKDPSAVQEYVVEWRELHPGGDTQVPLNWLRSRPYNVSALISENIKSYICYEIRVYALSGDQGGCSSILGNSKHKAPLSGPHINAITEEKGSILISWNSIPVQEQMGCLLHYRIYWKERDSNSQPQLCEIPYRVSQNSHPINSLQPRVTYVLWMTALTAAGESSHGNEREFCLQGK

Gene ID:3595
Swiss Prot:Q99665
Synonyms:IL12RB2
Calculated MW:74kDa/87kDa/97kDa
Observed MW:110kDa
Reactivity:Mouse, Rat
Application:WB
Recommended Dilution:WB 1:1000 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting
Positive Samples:Mouse brain, Rat brain
Cellular Location:Membrane, Single-pass type I membrane protein

Product Images

Anti-IL-12RB2 Antibody (CAB10348)

Western blot analysis of extracts of various cell lines, using IL12RB2 antibody (CAB10348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.

Uniprot Information

UniProt Protein Function:IL12RB2: Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production. Belongs to the type I cytokine receptor family. Type 2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Receptor, cytokine; Membrane protein, integral; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p31.3-p31.2

Cellular Component: integral to plasma membrane; external side of plasma membrane

Molecular Function:hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein kinase binding

Biological Process: positive regulation of interferon-gamma production; peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; cytokine and chemokine mediated signaling pathway; positive regulation of cell proliferation; interferon-gamma production; response to lipopolysaccharide

NCBI Summary:The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. Several transcript variants encoding different isoforms and non-protein coding transcripts have been found for this gene. [provided by RefSeq, Apr 2012]
UniProt Code:Q99665
NCBI GenInfo Identifier:12229836
NCBI Gene ID:3595
NCBI Accession:Q99665.1
UniProt Secondary Accession:Q99665,Q2M3V3, B1AN98, B7ZKL9, F5H7L6,
UniProt Related Accession:Q99665
Molecular Weight:87,484 Da
NCBI Full Name:Interleukin-12 receptor subunit beta-2
NCBI Synonym Full Names:interleukin 12 receptor, beta 2
NCBI Official Symbol:IL12RB2
NCBI Protein Information:interleukin-12 receptor subunit beta-2; IL-12R subunit beta-2; IL-12 receptor subunit beta-2; interleukin-12 receptor beta-2 chain
UniProt Protein Name:Interleukin-12 receptor subunit beta-2
Protein Family:Interleukin-12 receptor
UniProt Gene Name:IL12RB2
UniProt Entry Name:I12R2_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
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