Anti-KRT15 Antibody (CAB2660)

Antibody Name:	Anti-KRT15 Antibody
Antibody SKU:	CAB2660
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant protein of human KRT15

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human
Positive Samples:	TE-1, HeLa, SW480, OVCAR-3

Immunogen:	Recombinant protein of human KRT15
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	3866
Uniprot:	P19012
Cellular Location:
Calculated MW:	28kDa/49kDa
Observed MW:	47-50kDa

Synonyms:

KRT15, CK15, K15, K1CO

Background:

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2.

UniProt Protein Function:	K15: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.
UniProt Protein Details:	Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 17q21.2 Cellular Component: cytosol; nucleus Molecular Function:protein binding; structural constituent of cytoskeleton Biological Process: epidermis development; keratinization
NCBI Summary:	The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. [provided by RefSeq, Jul 2008]
UniProt Code:	P19012
NCBI GenInfo Identifier:	311033438
NCBI Gene ID:	3866
NCBI Accession:	P19012.3
UniProt Secondary Accession:	P19012,Q53XV8, Q9BUG4, B3KQY1, B3KRA2, E0CX14,
UniProt Related Accession:	P19012
Molecular Weight:	49kDa
NCBI Full Name:	Keratin, type I cytoskeletal 15
NCBI Synonym Full Names:	keratin 15
NCBI Official Symbol:	KRT15
NCBI Official Synonym Symbols:	K15; CK15; K1CO
NCBI Protein Information:	keratin, type I cytoskeletal 15
UniProt Protein Name:	Keratin, type I cytoskeletal 15
UniProt Synonym Protein Names:	Cytokeratin-15; CK-15; Keratin-15; K15
Protein Family:	Keratin
UniProt Gene Name:	KRT15

	Western blot analysis of extracts of various cell lines, using KRT15 antibody (CAB2660) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human colon carcinoma using KRT15 antibody (CAB2660) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney using KRT15 antibody (CAB2660) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using KRT15 antibody (CAB2660) at dilution of 1:200 (40x lens).

Antibodies	ELISA Kits
Anti-KRT15 Antibody (CAB12155)	Keratin 15 Colorimetric Cell-Based ELISA Kit
Anti-Cytokeratin 15 Antibody (CAB4854)
KRT15 Antibody (PACO06411)
KRT15 Antibody (PACO10162)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)