Anti-MVP Antibody (CAB1980)

Antibody Name:	Anti-MVP Antibody
Antibody SKU:	CAB1980
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human MVP (NP_005106.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human
Positive Samples:	HT-29, A-549, BT-474, HeLa, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human MVP (NP_005106.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATE EFII RIPP YHYI HVLD QNSN VSRV EVGP KTYI RQDN ERVL FAPM RMVT VPPR HYCT VANP VSRD AQGL VLFD VTGQ VRLR HADL EIRL AQDP FPLY PGEV LEKD ITPL QVVL PNTA LHLK ALLD FEDK DGDK VVAG DEWL FEGP GTYI PRKE VEVV EIIQ ATII RQNQ ALRL RARK ECWD RDGK ERVT GEEW LVTT VGAY LPAV FEEV LDLV DAVI LTEK TALH LRAR RNFR DFRG VSRR TGEE WLVT VQDT EAHV
Gene ID:	9961
Uniprot:	Q14764
Cellular Location:	Cytoplasm, Nucleus, nuclear pore complex
Calculated MW:	99kDa
Observed MW:	100kDa

Synonyms:

MVP, LRP, VAULT1

Background:

This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene.

UniProt Protein Function:	MVP: Required for normal vault structure. Vaults are multi- subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo- cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases.
UniProt Protein Details:	Protein type:RNA-binding Chromosomal Location of Human Ortholog: 16p11.2 Cellular Component: cytoskeleton; membrane; perinuclear region of cytoplasm; cytoplasm; nuclear pore; nucleus; ribonucleoprotein complex Molecular Function:protein binding; protein phosphatase binding; protein kinase binding Biological Process: cell proliferation; protein transport; mRNA transport; negative regulation of protein amino acid autophosphorylation
NCBI Summary:	This gene encodes the major component of the vault complex. Vaults are multi-subunit ribonucleoprotein structures that may be involved in nucleo-cytoplasmic transport. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT and phosphoinositide 3-kinase/Akt signaling pathways. The encoded protein also plays a role in multidrug resistance, and expression of this gene may be a prognostic marker for several types of cancer. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
UniProt Code:	Q14764
NCBI GenInfo Identifier:	21542417
NCBI Gene ID:	9961
NCBI Accession:	Q14764.4
UniProt Secondary Accession:	Q14764,Q96BG4, Q9BPW6, Q9BQT1, Q9UBD1,
UniProt Related Accession:	Q14764
Molecular Weight:	893
NCBI Full Name:	Major vault protein
NCBI Synonym Full Names:	major vault protein
NCBI Official Symbol:	MVP
NCBI Official Synonym Symbols:	LRP; VAULT1
NCBI Protein Information:	major vault protein; lung resistance-related protein
UniProt Protein Name:	Major vault protein
UniProt Synonym Protein Names:	Lung resistance-related protein
Protein Family:	Hyperpolarization-activated voltage-gated potassium channel
UniProt Gene Name:	MVP
UniProt Entry Name:	MVP_HUMAN

	Western blot analysis of extracts of various cell lines, using MVP antibody (CAB1980) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human esophagus using MVP Antibody (CAB1980) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human kidney cancer using MVP Antibody (CAB1980) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded human gastric cancer using MVP Antibody (CAB1980) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of U2OS cells using MVP antibody (CAB1980) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
MVP Antibody (PACO10665)	Human Major vault protein / MVP ELISA Kit
	Human LRP (Lung Resistance-Related Protein) ELISA Kit (HUES02329)
	Human Major vault protein (MVP) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)