The NPHS1 Antibody (CAB3048) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the nephrin protein, researchers can accurately detect and analyze its presence in various cell types, making it ideal for investigations in nephrology and kidney-related disorders.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
NPHS1 Antibody
SKU:
CAB3048
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CNF, NPHN, nephrin, NPHS1
Positive Sample:
Mouse kidney, mouse brain, rat brain, Rat kidney
Cellular Localization:
Cell Membrane, Single-Pass Type I Membrane Protein.
Calculated MW:
135kDa
Observed MW:
130kDa/180kDa
This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.
Purification Method
Affinity purification
Gene ID
4868
RRID
AB_2764852
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from mouse kidney, using NPHS1 Rabbit pAb (CAB3048) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
Western blot analysis of various lysates using NPHS1 Rabbit pAb (CAB3048) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
