Anti-KIF2C Antibody (CAB5449)

Antibody Name:	Anti-KIF2C Antibody
Antibody SKU:	CAB5449
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human KIF2C (NP_006836.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	THP-1, Lovo, K-562, HeLa, OVCAR3, U-937, U-251MG

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human KIF2C (NP_006836.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAMD SSLQ ARLF PGLA IKIQ RSNG LIHS ANVR TVNL EKSC VSVE WAEG GATK GKEI DFDD VAAI NPEL LQLL PLHP KDNL PLQE NVTI QKQK RRSV NSKI PAPK ESLR SRST RMST VSEL RITA QEND MEVE LPAA ANSR KQFS VPPA PTRP SCPA VAEI PLRM VSEE MEEQ VHSI RGSS SANP VNSV RR
Gene ID:	11004
Uniprot:	Q99661
Cellular Location:	Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, kinetochore
Calculated MW:	75kDa/81kDa
Observed MW:	95kDa

Synonyms:	KIF2C, CT139, KNSL6, MCAK
Background:	This gene encodes a kinesin-like protein that functions as a microtubule-dependent molecular motor. The encoded protein can depolymerize microtubules at the plus end, thereby promoting mitotic chromosome segregation. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	KIF2C: In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Cancer Testis Antigen (CTA); Motor; Microtubule-binding Chromosomal Location of Human Ortholog: 1p34.1 Cellular Component: chromosome, pericentric region; cytoplasmic microtubule; cytosol; kinesin complex; kinetochore; membrane; microtubule cytoskeleton Molecular Function:ATPase activity; centromeric DNA binding; microtubule plus-end binding; protein binding Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; cell proliferation; establishment and/or maintenance of microtubule cytoskeleton polarity; microtubule depolymerization; microtubule-based movement; mitosis; mitotic metaphase plate congression; regulation of chromosome segregation; retrograde vesicle-mediated transport, Golgi to ER; sister chromatid cohesion
NCBI Summary:	This gene encodes a kinesin-like protein that functions as a microtubule-dependent molecular motor. The encoded protein can depolymerize microtubules at the plus end, thereby promoting mitotic chromosome segregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Code:	Q99661
NCBI GenInfo Identifier:	20141607
NCBI Gene ID:	11004
NCBI Accession:	Q99661.2
UniProt Secondary Accession:	Q99661,Q5JR88, Q6ICU1, Q96C18, Q96HB8, Q9BWV8, B3ITR9
UniProt Related Accession:	Q99661
Molecular Weight:	75,561 Da
NCBI Full Name:	Kinesin-like protein KIF2C
NCBI Synonym Full Names:	kinesin family member 2C
NCBI Official Symbol:	KIF2C
NCBI Official Synonym Symbols:	MCAK; CT139; KNSL6
NCBI Protein Information:	kinesin-like protein KIF2C
UniProt Protein Name:	Kinesin-like protein KIF2C
UniProt Synonym Protein Names:	Kinesin-like protein 6; Mitotic centromere-associated kinesin; MCAK
Protein Family:	Kinesin-like protein
UniProt Gene Name:	KIF2C
UniProt Entry Name:	KIF2C_HUMAN

	Western blot analysis of extracts of various cell lines, using KIF2C antibody (CAB5449) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded Rat testis using KIF2C antibody (CAB5449) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Human stomach using KIF2C antibody (CAB5449) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Mouse testis using KIF2C antibody (CAB5449) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using KIF2C antibody (CAB5449). Blue: DAPI for nuclear staining.

Antibodies

Anti-MCAK Antibody (CAB9140)

Phospho-KIF2C (S95) Antibody (PACO03373)

KIF2C Antibody (PACO03374)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)