Anti-PPT2 Antibody (CAB12251)

Antibody Name:	Anti-PPT2 Antibody
Antibody SKU:	CAB12251
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 28-302 of human PPT2 (NP_005146.4).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 28-302 of human PPT2 (NP_005146.4).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	APAP HRAS YKPV IVVH GLFD SSYS FRHL LEYI NETH PGTV VTVL DLFD GRES LRPL WEQV QGFR EAVV PIMA KAPQ GVHL ICYS QGGL VCRA LLSV MDDH NVDS FISL SSPQ MGQY GDTD YLKW LFPT SMRS NLYR ICYS PWGQ EFSI CNYW HDPH HDDL YLNA SSFL ALIN GERD HPNA TVWR KNFL RVGH LVLI GGPD DGVI TPWQ SSFF GFYD ANET VLEM EEQL VYLR DSFG LKTL LARG AIVR CPMA GISH TAWH SNRT LYET CIEP WLS
Gene ID:	9374
Uniprot:	Q9UMR5
Cellular Location:	Lysosome
Calculated MW:	34kDa
Observed MW:	36kDa

Synonyms:

PPT2, C6orf8, G14, PPT-2

Background:

This gene encodes a member of the palmitoyl-protein thioesterase family. The encoded glycosylated lysosomal protein has palmitoyl-CoA hydrolase activity in vitro, but does not hydrolyze palmitate from cysteine residues in proteins. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream EGFL8 (EGF-like-domain, multiple 8) gene.

UniProt Protein Function:	PPT2: Removes thioester-linked fatty acyl groups from various substrates including S-palmitoyl-CoA. Has the highest S- thioesterase activity for the acyl groups palmitic and myristic acid followed by other short- and long-chain acyl substrates. However, because of structural constraints, is unable to remove palmitate from peptides or proteins. Belongs to the palmitoyl-protein thioesterase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.1.2.-; Hydrolase; Lipid Metabolism - fatty acid elongation in mitochondria Chromosomal Location of Human Ortholog: 6p21.32 Cellular Component: lysosomal lumen
NCBI Summary:	This gene encodes a member of the palmitoyl-protein thioesterase family. The encoded glycosylated lysosomal protein has palmitoyl-CoA hydrolase activity in vitro, but does not hydrolyze palmitate from cysteine residues in proteins. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the downstream EGFL8 (EGF-like-domain, multiple 8) gene. [provided by RefSeq, Feb 2011]
UniProt Code:	Q9UMR5
NCBI GenInfo Identifier:	296453016
NCBI Gene ID:	9374
NCBI Accession:	Q9UMR5.4
UniProt Secondary Accession:	Q9UMR5,O14799, Q0P6K0, A2ABC9, A2ABD1, A2ARM7, A2BFH7 A2BFH9, A2BFI2, A8K9L4, B0S868, G8JLE1,
UniProt Related Accession:	Q9UMR5
Molecular Weight:	34kDa
NCBI Full Name:	Lysosomal thioesterase PPT2
NCBI Synonym Full Names:	palmitoyl-protein thioesterase 2
NCBI Official Symbol:	PPT2
NCBI Official Synonym Symbols:	G14; PPT-2; C6orf8
NCBI Protein Information:	lysosomal thioesterase PPT2
UniProt Protein Name:	Lysosomal thioesterase PPT2
UniProt Synonym Protein Names:	S-thioesterase G14
Protein Family:	Phosphoenolpyruvate/phosphate translocator
UniProt Gene Name:	PPT2

	Western blot analysis of extracts of mouse liver, using PPT2 antibody (CAB12251) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse heart using PPT2 antibody (CAB12251) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)