Anti-Wnt5a Antibody (CAB2133)

Antibody Name:	Anti-Wnt5a Antibody
Antibody SKU:	CAB2133
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 101-380 of human Wnt5a (NP_003383.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SKOV3, HeLa, Mouse lung, Mouse kidney, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 101-380 of human Wnt5a (NP_003383.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IKEC QYQF RHRR WNCS TVDN TSVF GRVM QIGS RETA FTYA VSAA GVVN AMSR ACRE GELS TCGC SRAA RPKD LPRD WLWG GCGD NIDY GYRF AKEF VDAR ERER IHAK GSYE SARI LMNL HNNE AGRR TVYN LADV ACKC HGVS GSCS LKTC WLQL ADFR KVGD ALKE KYDS AAAM RLNS RGKL VQVN SRFN SPTT QDLV YIDP SPDY CVRN ESTG SLGT QGRL CNKT SEGM DGCE LMCC GRGY DQFK TVQT ERCH CKFH WCCY VKCK KCTE IVDQ FVCK
Gene ID:	7474
Uniprot:	P41221
Cellular Location:	Secreted, extracellular matrix, extracellular space
Calculated MW:	40kDa/42kDa
Observed MW:	42KDa

Synonyms:

hWNT5A, Wnt5a, WNT5A

Background:

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	WNT5A: Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes. Interacts with PORCN. Interacts with WLS. Expression is increased in differentiated thyroid carcinomas compared to normal thyroid tissue and anaplastic thyroid tumors where expression is low or undetectable. Expression is found in thyrocytes but not in stromal cells. Belongs to the Wnt family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 3p14.3 Cellular Component: clathrin coated vesicle membrane; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen; plasma membrane Molecular Function:frizzled binding; protein binding; receptor agonist activity; receptor tyrosine kinase-like orphan receptor binding; transcription factor activity Biological Process: activation of JNK activity; activation of MAPK activity; activation of NF-kappaB transcription factor; activation of protein kinase B; axon guidance; cell fate commitment; embryonic skeletal development; epithelial to mesenchymal transition; genitalia development; keratinocyte differentiation; lens development in camera-type eye; male gonad development; negative regulation of apoptosis; negative regulation of fat cell differentiation; negative regulation of transcription, DNA-dependent; neuron differentiation; olfactory bulb interneuron development; palate development; positive regulation of angiogenesis; positive regulation of cGMP metabolic process; positive regulation of chemokine biosynthetic process; positive regulation of cytokine secretion during immune response; positive regulation of endothelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of inflammatory response; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-6 production; positive regulation of macrophage activation; positive regulation of ossification; positive regulation of protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; response to organic substance; Wnt receptor signaling pathway; Wnt receptor signaling pathway, calcium modulating pathway; Wnt receptor signaling pathway, planar cell polarity pathway; wound healing Disease: Robinow Syndrome, Autosomal Dominant
NCBI Summary:	The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene encodes a member of the WNT family that signals through both the canonical and non-canonical WNT pathways. This protein is a ligand for the seven transmembrane receptor frizzled-5 and the tyrosine kinase orphan receptor 2. This protein plays an essential role in regulating developmental pathways during embryogenesis. This protein may also play a role in oncogenesis. Mutations in this gene are the cause of autosomal dominant Robinow syndrome. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
UniProt Code:	P41221
NCBI GenInfo Identifier:	212276478
NCBI Gene ID:	7474
NCBI Accession:	P41221.2
UniProt Secondary Accession:	P41221,Q6P278, A8K4A4,
UniProt Related Accession:	P41221
Molecular Weight:	42kDa
NCBI Full Name:	Protein Wnt-5a
NCBI Synonym Full Names:	Wnt family member 5A
NCBI Official Symbol:	WNT5A
NCBI Official Synonym Symbols:	hWNT5A
NCBI Protein Information:	protein Wnt-5a
UniProt Protein Name:	Protein Wnt-5a
Protein Family:	Protein
UniProt Gene Name:	WNT5A

	Western blot analysis of extracts of various cell lines, using Wnt5a Polyclonal Antibody (CAB2133) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
	Immunohistochemistry of paraffin-embedded human prostate using Wnt5a antibody (CAB2133) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using Wnt5a antibody (CAB2133) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-WNT5A Antibody (CAB12744)	Human WNT5A ELISA Kit
Anti-Wnt5a Antibody (CAB19133)	Human Protein Wnt-5a (WNT5A) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)