Anti-RHO Antibody (CAB15093)

Antibody Name:	Anti-RHO Antibody
Antibody SKU:	CAB15093
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human RHO (NP_000530.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	mouse eye, rat eye

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human RHO (NP_000530.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NESF VIYM FVVH FTIP MIII FFCY GQLV FTVK EAAA QQQE SATT QKAE KEVT RMVI IMVI AFLI CWVP YASV AFYI FTHQ GSNF GPIF MTIP AFFA KSAA IYNP VIYI MMNK QFRN CMLT TICC GKNP LGDD EASA TVSK TETS QVAP A
Gene ID:	6010
Uniprot:	P08100
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	38kDa
Observed MW:	39kDa

Synonyms:

RHO, CSNBAD1, OPN2, RP4, rhodopsin

Background:

Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form, which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness.

UniProt Protein Function:	Rhodopsin: a G-protein coupled receptor. The light-absorbing visual pigment in Rod photoreceptor cells. Mediates vision in dim light. Consists of the apoprotein, opsin, covalently linked to cis-retinal. Defects in RHO are a cause of autosomal retinitis pigmentosa and congenital stationary night blindness.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1 Chromosomal Location of Human Ortholog: 3q21-q24 Cellular Component: Golgi apparatus; Golgi membrane; integral to plasma membrane; photoreceptor inner segment; photoreceptor outer segment; plasma membrane Molecular Function:G-protein coupled photoreceptor activity; G-protein coupled receptor activity; protein binding Biological Process: absorption of visible light; G-protein coupled receptor protein signaling pathway; phototransduction, visible light; regulation of rhodopsin mediated signaling; retinoid metabolic process; rhodopsin mediated signaling Disease: Fundus Albipunctatus; Night Blindness, Congenital Stationary, Autosomal Dominant 1; Retinitis Pigmentosa 4
NCBI Summary:	Retinitis pigmentosa is an inherited progressive disease which is a major cause of blindness in western communities. It can be inherited as an autosomal dominant, autosomal recessive, or X-linked recessive disorder. In the autosomal dominant form,which comprises about 25% of total cases, approximately 30% of families have mutations in the gene encoding the rod photoreceptor-specific protein rhodopsin. This is the transmembrane protein which, when photoexcited, initiates the visual transduction cascade. Defects in this gene are also one of the causes of congenital stationary night blindness. [provided by RefSeq, Jul 2008]
UniProt Code:	P08100
NCBI GenInfo Identifier:	129207
NCBI Gene ID:	6010
NCBI Accession:	P08100.1
UniProt Secondary Accession:	P08100,Q16414, Q2M249,
UniProt Related Accession:	P08100
Molecular Weight:	38,893 Da
NCBI Full Name:	Rhodopsin
NCBI Synonym Full Names:	rhodopsin
NCBI Official Symbol:	RHO
NCBI Official Synonym Symbols:	RP4; OPN2; CSNBAD1
NCBI Protein Information:	rhodopsin
UniProt Protein Name:	Rhodopsin
UniProt Synonym Protein Names:	Opsin-2
Protein Family:	Rhotekin
UniProt Gene Name:	RHO
UniProt Entry Name:	OPSD_HUMAN

	Western blot analysis of extracts of various cell lines, using RHO antibody (CAB15093) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded mouse retina using RHO antibody (CAB15093) at dilution of 1:200 (40x lens).

Antibodies	ELISA Kits
RHO Antibody (PACO01432)	Human Rhodopsin (RHO) ELISA Kit
RHO Antibody (PACO01433)	Rhodopsin Colorimetric Cell-Based ELISA Kit
Phospho-RHO (S334) Antibody (PACO04923)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)