The SETD2 Antibody (CAB3194) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it ideal for use in Western blot applications.SETD2 is a critical player in maintaining genomic stability and proper gene expression through its histone-modifying activity. Dysregulation of SETD2 has been linked to various cancers, highlighting its importance in tumorigenesis and potential as a therapeutic target.
This antibody is validated for use in WB, IHC-P, IF/ICC, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
SETD2 Antibody
SKU:
CAB3194
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II.
Purification Method
Affinity purification
Gene ID
29072
RRID
AB_2764980
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Mouse spleen, using SETD2 Rabbit pAb (CAB3194) at 1:900 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using SETD2 Rabbit pAb (CAB3194) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using SETD2 Rabbit pAb (CAB3194) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using SETD2 Rabbit pAb (CAB3194) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of U-2 OS cells using SETD2 Rabbit pAb (CAB3194) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 600 μg extracts of Mouse spleen using 3 μg SETD2 antibody (CAB3194). Western blot was performed from the immunoprecipitate using SETD2 antibody (CAB3194) at a dilution of 1:1000.
