Anti-TRAF3IP2 Antibody (CAB6776)

Antibody Name:	Anti-TRAF3IP2 Antibody
Antibody SKU:	CAB6776
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TRAF3IP2 (NP_679211.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, BT-474, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human TRAF3IP2 (NP_679211.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNRS IPVE VDES EPYP SQLL KPIP EYSP EEES EPPA PNIR NMAP NSLS APTM LHNS SGDF SQAH STLK LANH QRPV SRQV TCLR TQVL EDSE DSFC RRHP GLGK AFPS GCSA VSEP ASES VVGA LPAE HQFS FMEK RNQW LVSQ LSAA SPDT GHDS DKSD QSLP NASA DSLG GSQE MVQR PQPH RNRA GLDL PTID TGYD SQPQ DVLG IRQL ERPL PLTS VCYP QDLP RPLR SREF PQFE PQRY PACA QMLP PNLS PHAP WNYH YHCP GSPD HQVP YGHD YPRA AYQQ VIQP ALPG QPLP
Gene ID:	10758
Uniprot:	O43734
Cellular Location:
Calculated MW:	12kDa/18kDa/63kDa/64kDa
Observed MW:	70kDa

Synonyms:

TRAF3IP2, ACT1, C6orf2, C6orf4, C6orf5, C6orf6, CANDF8, CIKS, PSORS13

Background:

This gene encodes a protein involved in regulating responses to cytokines by members of the Rel/NF-kappaB transcription factor family. These factors play a central role in innate immunity in response to pathogens, inflammatory signals and stress. This gene product interacts with TRAF proteins (tumor necrosis factor receptor-associated factors) and either I-kappaB kinase or MAP kinase to activate either NF-kappaB or Jun kinase. Several alternative transcripts encoding different isoforms have been identified. Another transcript, which does not encode a protein and is transcribed in the opposite orientation, has been identified. Overexpression of this transcript has been shown to reduce expression of at least one of the protein encoding transcripts, suggesting it has a regulatory role in the expression of this gene.

UniProt Protein Function:	TRAF3IP2: Could be involved in the activation of both NF-kappa-B via a NF-kappa-B inhibitor kinase (IKK)-dependent mechanism and stress-activated protein kinase (SAPK)/JNK. Defects in TRAF3IP2 are the cause of susceptibility to psoriasis type 13 (PSORS13). PSORS13 is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Apoptosis Chromosomal Location of Human Ortholog: 6q21 Cellular Component: intracellular Molecular Function:protein binding; receptor binding Biological Process: B cell apoptosis; humoral immune response; immunoglobulin secretion; positive regulation of defense response to virus by host; positive regulation of I-kappaB kinase/NF-kappaB cascade Disease: Candidiasis, Familial, 8; Psoriasis 13, Susceptibility To
NCBI Summary:	This gene encodes a protein involved in regulating responses to cytokines by members of the Rel/NF-kappaB transcription factor family. These factors play a central role in innate immunity in response to pathogens, inflammatory signals and stress. This gene product interacts with TRAF proteins (tumor necrosis factor receptor-associated factors) and either I-kappaB kinase or MAP kinase to activate either NF-kappaB or Jun kinase. Several alternative transcripts encoding different isoforms have been identified. Another transcript, which does not encode a protein and is transcribed in the opposite orientation, has been identified. Overexpression of this transcript has been shown to reduce expression of at least one of the protein encoding transcripts, suggesting it has a regulatory role in the expression of this gene. [provided by RefSeq, Aug 2009]
UniProt Code:	O43734
NCBI GenInfo Identifier:	317373453
NCBI Gene ID:	10758
NCBI Accession:	O43734.3
UniProt Secondary Accession:	O43734,Q5R3A3, Q7Z6Q1, Q7Z6Q2, Q7Z6Q3, Q9H5W2, Q9H6Y3 Q9NS14, Q9UG72, B2RAY9, E1P555,
UniProt Related Accession:	O43734
Molecular Weight:	63,457 Da
NCBI Full Name:	Adapter protein CIKS
NCBI Synonym Full Names:	TRAF3 interacting protein 2
NCBI Official Symbol:	TRAF3IP2
NCBI Official Synonym Symbols:	ACT1; CIKS; C6orf2; C6orf4; C6orf5; C6orf6; CANDF8; PSORS13
NCBI Protein Information:	adapter protein CIKS
UniProt Protein Name:	Adapter protein CIKS
UniProt Synonym Protein Names:	Connection to IKK and SAPK/JNK; Nuclear factor NF-kappa-B activator 1; ACT1; TRAF3-interacting protein 2
UniProt Gene Name:	TRAF3IP2
UniProt Entry Name:	CIKS_HUMAN

	Western blot analysis of extracts of various cell lines, using TRAF3IP2 antibody (CAB6776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver damage using TRAF3IP2 Antibody (CAB6776) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat spleen using TRAF3IP2 Antibody (CAB6776) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)