Anti-WRB Antibody (CAB7000)

Antibody Name:	Anti-WRB Antibody
Antibody SKU:	CAB7000
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-100 of human WRB (NP_004618.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse testis, Mouse skin, Rat lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-100 of human WRB (NP_004618.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SFSS FMSR VLQK DAEQ ESQM RAEI QDMK QELS TVNM MDEF ARYA RLER KINK MTDK LKTH VKAR TAQL AKI
Gene ID:	7485
Uniprot:	O00258
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	16kDa/19kDa
Observed MW:	25kDa

Synonyms:

WRB, CHD5, GET1

Background:

This gene encodes a basic nuclear protein of unknown function. The gene is widely expressed in adult and fetal tissues. Since the region proposed to contain the gene(s) for congenital heart disease (CHD) in Down syndrome (DS) patients has been restricted to 21q22.2-22.3, this gene, which maps to 21q22.3, has a potential role in the pathogenesis of Down syndrome congenital heart disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	WRB: Receptor for ASNA1/TRC40-mediated insertion of tail- anchored (TA) proteins into the ER membrane. Belongs to the WRB/GET1 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: endoplasmic reticulum membrane; integral to membrane; nucleus
NCBI Summary:	This gene encodes a basic nuclear protein of unknown function. The gene is widely expressed in adult and fetal tissues. Since the region proposed to contain the gene(s) for congenital heart disease (CHD) in Down syndrome (DS) patients has been restricted to 21q22.2-22.3, this gene, which maps to 21q22.3, has a potential role in the pathogenesis of Down syndrome congenital heart disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]
UniProt Code:	O00258
NCBI GenInfo Identifier:	12643986
NCBI Gene ID:	7485
NCBI Accession:	O00258.2
UniProt Secondary Accession:	O00258,O60740, A8KAP8, A8MQ44, D3DSH9,
UniProt Related Accession:	O00258
Molecular Weight:	174
NCBI Full Name:	Tail-anchored protein insertion receptor WRB
NCBI Synonym Full Names:	tryptophan rich basic protein
NCBI Official Symbol:	WRB
NCBI Official Synonym Symbols:	CHD5
NCBI Protein Information:	tail-anchored protein insertion receptor WRB; tryptophan-rich protein; tryptophan-rich basic protein; congenital heart disease 5 protein
UniProt Protein Name:	Tail-anchored protein insertion receptor WRB
UniProt Synonym Protein Names:	Congenital heart disease 5 protein; Tryptophan-rich basic protein; WRB
Protein Family:	Tail-anchored protein insertion receptor
UniProt Gene Name:	WRB
UniProt Entry Name:	WRB_HUMAN

	Western blot analysis of extracts of various cell lines, using WRB antibody (CAB7000) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat kidney using WRB antibody (CAB7000) at dilution of 1:200 (40x lens).

ELISA Kits

Human CHD5 / Chromodomain Helicase DNA Binding Protein 5 ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)