Anti-WSB2 Antibody (CAB12970)

Antibody Name:	Anti-WSB2 Antibody
Antibody SKU:	CAB12970
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human WSB2 (NP_061109.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Mouse, Rat
Positive Samples:	Mouse kidney, Rat kidney, Rat spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human WSB2 (NP_061109.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEAG EEPL LLAE LKPG RPHQ FDWK SSCE TWSV AFSP DGSW FAWS QGHC IVKL IPWP LEEQ FIPK GFEA KSRS SKNE TKGR GSPK EKTL DCGQ IVWG LAFS PWPS PPSR KLWA RHHP QVPD VSCL VLAT GLND GQIK IWEV QTGL LLLN LSGH QDVV RDLS FTPS GSLI LVSA SRDK TLRI WDLN KHGK QIQV LSGH LQWV
Gene ID:	55884
Uniprot:	Q9NYS7
Cellular Location:
Calculated MW:	21kDa/45kDa/47kDa
Observed MW:	40-43kDa

Synonyms:	WSB2, SBA2
Background:	This gene encodes a member of the WD-protein subfamily. The encoded protein contains five WD-repeats spanning most of the protein and an SOCS box in the C-terminus. The SOCS box may act as a bridge between specific substrate-binding domains and E3 ubiquitin protein ligases. Three transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	WSB2: May be a substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
UniProt Protein Details:	Protein type:Unknown function Chromosomal Location of Human Ortholog: 12q24.23
NCBI Summary:	This gene encodes a member of the WD-protein subfamily. The encoded protein contains five WD-repeats spanning most of the protein and an SOCS box in the C-terminus. The SOCS box may act as a bridge between specific substrate-binding domains and E3 ubiquitin protein ligases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]
UniProt Code:	Q9NYS7
NCBI GenInfo Identifier:	20532294
NCBI Gene ID:	55884
NCBI Accession:	Q9NYS7.1
UniProt Secondary Accession:	Q9NYS7,Q9NRX9, B4DIE6, B4DPV6,
UniProt Related Accession:	Q9NYS7
Molecular Weight:	21,809 Da
NCBI Full Name:	WD repeat and SOCS box-containing protein 2
NCBI Synonym Full Names:	WD repeat and SOCS box containing 2
NCBI Official Symbol:	WSB2
NCBI Official Synonym Symbols:	SBA2
NCBI Protein Information:	WD repeat and SOCS box-containing protein 2
UniProt Protein Name:	WD repeat and SOCS box-containing protein 2
UniProt Synonym Protein Names:	CS box-containing WD protein
Protein Family:	WD repeat and SOCS box-containing protein
UniProt Gene Name:	WSB2
UniProt Entry Name:	WSB2_HUMAN

	Western blot analysis of extracts of various cell lines, using WSB2 antibody (CAB12970) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat heart using WSB2 antibody (CAB12970) at dilution of 1:100 (40x lens).

Secondary Antibody