Anti-ZFYVE16 Antibody (CAB7766)

Antibody Name:	Anti-ZFYVE16 Antibody
Antibody SKU:	CAB7766
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ZFYVE16 (NP_055548.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ZFYVE16 (NP_055548.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDSY FKAA VSDL DKLL DDFE QNPD EQDY LQDV QNAY DSNH CSVS SELA SSQR TSLL PKDQ ECVN SCAS SETS YGTN ESSL NEKT LKGL TSIQ NEKN VTGL DLLS SVDG GTSD EIQP LYMG RCSK PICD LISD MGNL VHAT NSEE DIKK LLPD DFKS NADS LIGL DLSS VSDT PCVS STDH DSDT VREQ QNDI SSEL QNRE IGGI KELG IKVD TTLS DSYN YSGT ENLK DKKI FNQL ESIV DFNM SSAL TRQS SKMF HAKD KLQH KSQP CGLL KDVG LVKE
Gene ID:	9765
Uniprot:	Q7Z3T8
Cellular Location:	Cytoplasm, Early endosome membrane, Peripheral membrane protein
Calculated MW:	88kDa/168kDa
Observed MW:	100kDa

Synonyms:

ZFYVE16, PPP1R69

Background:

This gene encodes an endosomal protein that belongs to the FYVE zinc finger family of proteins. The encoded protein is thought to regulate membrane trafficking in the endosome. This protein functions as a scaffold protein in the transforming growth factor-beta signaling pathway and is involved in positive and negative feedback regulation of the bone morphogenetic protein signaling pathway. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	endofin: May be involved in regulating membrane trafficking in the endosomal pathway. Overexpression induces endosome aggregation. Required to target TOM1 to endosomes. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Lipid-binding Chromosomal Location of Human Ortholog: 5q14 Cellular Component: cytoplasm; early endosome; early endosome membrane; intracellular membrane-bound organelle Molecular Function:phosphatidylinositol binding; phosphatidylinositol-3,4,5-triphosphate binding; protein binding; protein transporter activity Biological Process: BMP signaling pathway; endosome transport; protein targeting to lysosome; regulation of endocytosis
NCBI Summary:	This gene encodes an endosomal protein that belongs to the FYVE zinc finger family of proteins. The encoded protein is thought to regulate membrane trafficking in the endosome. This protein functions as a scaffold protein in the transforming growth factor-beta signaling pathway and is involved in positive and negative feedback regulation of the bone morphogenetic protein signaling pathway. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]
UniProt Code:	Q7Z3T8
NCBI GenInfo Identifier:	296453075
NCBI Gene ID:	9765
NCBI Accession:	Q7Z3T8.3
UniProt Secondary Accession:	Q7Z3T8,O15023, Q5H9U2, Q7LAU7, Q86T69, Q8N5L3, Q8NEK3
UniProt Related Accession:	Q7Z3T8
Molecular Weight:	88,377 Da
NCBI Full Name:	Zinc finger FYVE domain-containing protein 16
NCBI Synonym Full Names:	zinc finger FYVE-type containing 16
NCBI Official Symbol:	ZFYVE16
NCBI Official Synonym Symbols:	PPP1R69
NCBI Protein Information:	zinc finger FYVE domain-containing protein 16
UniProt Protein Name:	Zinc finger FYVE domain-containing protein 16
UniProt Synonym Protein Names:	Endofin; Endosome-associated FYVE domain protein
Protein Family:	Zinc finger FYVE domain-containing protein
UniProt Gene Name:	ZFYVE16
UniProt Entry Name:	ZFY16_HUMAN

	Western blot analysis of extracts of mouse kidney, using ZFYVE16 antibody (CAB7766) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using ZFYVE16 antibody (CAB7766) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)