Anti-ABCG8 Antibody (CAB1880)

Antibody Name:	Anti-ABCG8 Antibody
Antibody SKU:	CAB1880
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human ABCG8 (NP_071882.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human ABCG8 (NP_071882.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAGK AAEE RGLP KGAT PQDT SGLQ DRLF SSES DNSL YFTY SGQP NTLE VRDL NYQV DLAS QVPW FEQL AQFK MPWT SPSC QNSC ELGI QNLS FKVR SGQM LAII GSSG CGRA SLLD VITG RGHG GKIK SGQI WING QPSS PQLV RKCV AHVR QHNQ LLPN LTVR ETLA FIAQ MRLP RTFS QAQR DKRV EDVI AELR LRQC ADTR VGNM YVRG LSGG ERRR VSIG VQLL WNPG ILIL DEPT SGLD SFTA HNLV KTLS RLAK GNRL VLIS LHQP RSDI FRLF DLVL LMTS GTPI YLGA AQHM VQYF TAIG YPCP RYSN PADF
Gene ID:	64241
Uniprot:	Q9H221
Cellular Location:	Membrane, Multi-pass membrane protein
Calculated MW:	75kDa
Observed MW:	76kDa

Synonyms:

ABCG8, GBD4, STSL

Background:

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions to exclude non-cholesterol sterol entry at the intestinal level, promote excretion of cholesterol and sterols into bile, and to facilitate transport of sterols back into the intestinal lumen. It is expressed in a tissue-specific manner in the liver, intestine, and gallbladder. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG5. Mutations in this gene may contribute to sterol accumulation and atherosclerosis, and have been observed in patients with sitosterolemia.

UniProt Protein Function:	ABCG8: Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Genetic variations in ABCG8 can be associated with susceptibility to gallbladder disease type 4 (GBD4). With an overall prevalence of 10-20%, gallstone disease (cholelithiasis) represents one of the most frequent and economically relevant health problems of industrialized countries. Defects in ABCG8 are a cause of sitosterolemia (STSL); also known as phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral; Transporter, ABC family; Transporter Chromosomal Location of Human Ortholog: 2p21 Cellular Component: apical plasma membrane; plasma membrane; ATP-binding cassette (ABC) transporter complex; receptor complex Molecular Function:protein binding; protein heterodimerization activity; cholesterol transporter activity; ATPase activity; ATP binding Biological Process: response to drug; negative regulation of cholesterol absorption; cholesterol homeostasis; cholesterol absorption; phospholipid transport; cholesterol efflux; excretion; transmembrane transport; response to nutrient; sterol transport Disease: Sitosterolemia; Gallbladder Disease 4
NCBI Summary:	The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions to exclude non-cholesterol sterol entry at the intestinal level, promote excretion of cholesterol and sterols into bile, and to facilitate transport of sterols back into the intestinal lumen. It is expressed in a tissue-specific manner in the liver, intestine, and gallbladder. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG5. Mutations in this gene may contribute to sterol accumulation and atherosclerosis, and have been observed in patients with sitosterolemia. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9H221
NCBI GenInfo Identifier:	17432916
NCBI Gene ID:	64241
NCBI Accession:	Q9H221.1
UniProt Secondary Accession:	Q9H221,Q9DBM0, P58428,
UniProt Related Accession:	Q9H221
Molecular Weight:	673
NCBI Full Name:	ATP-binding cassette sub-family G member 8
NCBI Synonym Full Names:	ATP-binding cassette, sub-family G (WHITE), member 8
NCBI Official Symbol:	ABCG8
NCBI Official Synonym Symbols:	GBD4; STSL
NCBI Protein Information:	ATP-binding cassette sub-family G member 8; sterolin 2; sterolin-2; ATP-binding cassette, subfamily G, member 8
UniProt Protein Name:	ATP-binding cassette sub-family G member 8
UniProt Synonym Protein Names:	Sterolin-2
Protein Family:	ABC transporter G family
UniProt Gene Name:	ABCG8
UniProt Entry Name:	ABCG8_HUMAN

	Western blot analysis of extracts of A-549 cells, using ABCG8 antibody (CAB1880). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of HeLa cells using ABCG8 antibody (CAB1880) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)