Anti-Tubulin alpha 1b Antibody (CABC025)

SKU:
CABC025
  • Loading Controls Anti-Tubulin alpha 1b Antibody CABC025
  • Loading Controls Anti-Tubulin alpha 1b Antibody CABC025
  • Loading Controls Anti-Tubulin alpha 1b Antibody CABC025
  • Loading Controls Anti-Tubulin alpha 1b Antibody CABC025
€299

Description

Technical Manual

Anti-Tubulin alpha 1b Antibody

Product Name:Anti-Tubulin alpha 1b Antibody
Product SKU:CABC025
Size:100 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-451 of human Tubulin alpha 1b (NP_006073.2).
Sequence:

MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY

Gene ID:10376
Swiss Prot:P68363
Synonyms:TUBA1B; K-ALPHA-1
Calculated MW:37kDa/50kDa
Observed MW:50KDa
Reactivity:Human, Mouse, Rat
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting Immunofluorescence 
Positive Samples:HeLa, PC-12, A-549, NIH/3T3, U-251MG, Mouse brain , Rat brain
Cellular Location:Cytoplasm, cytoskeleton

Product Images

Anti-Tubulin alpha 1b Antibody (CABC025)

Western blot analysis of extracts of various cell lines, using Tubulin alpha 1b antibody (CABC025) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Anti-Tubulin alpha 1b Antibody (CABC025)

Immunofluorescence analysis of U2OS cells using Tubulin alpha 1b antibody (CABC025) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-Tubulin alpha 1b Antibody (CABC025)

Immunofluorescence analysis of NIH/3T3 cells using Tubulin alpha 1b antibody (CABC025) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-Tubulin alpha 1b Antibody (CABC025)

Immunofluorescence analysis of C6 cells using Tubulin alpha 1b antibody (CABC025) at dilution of 1:100. Blue: DAPI for nuclear staining.

Uniprot Information

UniProt Protein Function:TUBA1B: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.
UniProt Protein Details:

Protein type:Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q13.12

Cellular Component: microtubule; cytoplasmic microtubule

Molecular Function:GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton; ubiquitin protein ligase binding; double-stranded RNA binding; structural molecule activity

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; cell division; cytoskeleton-dependent intracellular transport; microtubule cytoskeleton organization and biogenesis; microtubule-based process

UniProt Code:P68363
NCBI GenInfo Identifier:55977474
NCBI Gene ID:10376
NCBI Accession:P68363.1
UniProt Secondary Accession:P68363,P68366,
UniProt Related Accession:P68363
Molecular Weight:55kDa
NCBI Full Name:Tubulin alpha-1B chain
NCBI Synonym Full Names:tubulin alpha 1b
NCBI Official Symbol:TUBA1B 
NCBI Official Synonym Symbols:K-ALPHA-1 
NCBI Protein Information:tubulin alpha-1B chain
UniProt Protein Name:Tubulin alpha-1B chain
UniProt Synonym Protein Names:Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain
Protein Family:Tubulin
UniProt Gene Name:TUBA1B 
UniProt Entry Name:TBA1B_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
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