Anti-Tubulin alpha 1b Antibody (CABC025)
- SKU:
- CABC025
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
Frequently bought together:
Description
| Antibody Name: | Anti-Tubulin alpha 1b Antibody |
| Antibody SKU: | CABC025 |
| Antibody Size: | 100 uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-451 of human Tubulin alpha 1b (NP_006073.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, PC-12, A-549, NIH/3T3, U-251MG, Mouse brain , Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-451 of human Tubulin alpha 1b (NP_006073.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MREC ISIH VGQA GVQI GNAC WELY CLEH GIQP DGQM PSDK TIGG GDDS FNTF FSET GAGK HVPR AVFV DLEP TVID EVRT GTYR QLFH PEQL ITGK EDAA NNYA RGHY TIGK EIID LVLD RIRK LADQ CTGL QGFL VFHS FGGG TGSG FTSL LMER LSVD YGKK SKLE FSIY PAPQ VSTA VVEP YNSI LTTH TTLE HSDC AFMV DNEA IYDI CRRN LDIE RPTY TNLN RLIS QIVS SITA SLRF DGAL NVDL TEFQ TNLV PYPR IHFP LATY APVI SAEK AYHE QLSV AEIT NACF EPAN QMVK CDPR HGKY MACC LLYR GDVV PKDV NAAI ATIK TKRS IQFV DWCP TGFK VGIN YQPP TVVP GGDL AKVQ RAVC MLSN TTAI AEAW ARLD HKFD LMYA KRAF VHWY VGEG MEEG EFSE ARED MAAL EKDY EEVG VDSV EGEG EEEG EEY |
| Gene ID: | 10376 |
| Uniprot: | P68363 |
| Cellular Location: | Cytoplasm, cytoskeleton |
| Calculated MW: | 37kDa/50kDa |
| Observed MW: | 50KDa |
 | Western blot analysis of extracts of various cell lines, using Tubulin alpha 1b antibody (CABC025) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of U2OS cells using Tubulin alpha 1b antibody (CABC025) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using Tubulin alpha 1b antibody (CABC025) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of C6 cells using Tubulin alpha 1b antibody (CABC025) at dilution of 1:100. Blue: DAPI for nuclear staining. |
| Synonyms: | TUBA1B, K-ALPHA-1 |
| Background: | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. |
| UniProt Protein Function: | TUBA1B: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 12q13.12 Cellular Component: microtubule; cytoplasmic microtubule Molecular Function:GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton; ubiquitin protein ligase binding; double-stranded RNA binding; structural molecule activity Biological Process: protein polymerization; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; cell division; cytoskeleton-dependent intracellular transport; microtubule cytoskeleton organization and biogenesis; microtubule-based process |
| UniProt Code: | P68363 |
| NCBI GenInfo Identifier: | 55977474 |
| NCBI Gene ID: | 10376 |
| NCBI Accession: | P68363.1 |
| UniProt Secondary Accession: | P68363,P68366, |
| UniProt Related Accession: | P68363 |
| Molecular Weight: | 55kDa |
| NCBI Full Name: | Tubulin alpha-1B chain |
| NCBI Synonym Full Names: | tubulin alpha 1b |
| NCBI Official Symbol: | TUBA1B |
| NCBI Official Synonym Symbols: | K-ALPHA-1 |
| NCBI Protein Information: | tubulin alpha-1B chain |
| UniProt Protein Name: | Tubulin alpha-1B chain |
| UniProt Synonym Protein Names: | Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain |
| Protein Family: | Tubulin |
| UniProt Gene Name: | TUBA1B |
| UniProt Entry Name: | TBA1B_HUMAN |
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