Anti-ANXA7 Antibody (CAB3733)

Antibody Name:	Anti-ANXA7 Antibody
Antibody SKU:	CAB3733
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 237-466 of human ANXA7 (NP_001147.1).

Application:	WB IF
Recommended Dilution:	WB 1:200 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, NCI-H460, HepG2, SW480, NIH/3T3, Mouse heart, Mouse lung, Rat lung

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 237-466 of human ANXA7 (NP_001147.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TYYD AWSL RKAM QGAG TQER VLIE ILCT RTNQ EIRE IVRC YQSE FGRD LEKD IRSD TSGH FERL LVSM CQGN RDEN QSIN HQMA QEDA QRLY QAGE GRLG TDES CFNM ILAT RSFP QLRA TMEA YSRM ANRD LLSS VSRE FSGY VESG LKTI LQCA LNRP AFFA ERLY YAMK GAGT DDST LVRI VVTR SEID LVQI KQMF AQMY QKTL GTMI AGDT SGDY RRLL LAIV GQ
Gene ID:	310
Uniprot:	P20073
Cellular Location:
Calculated MW:	50kDa/52kDa
Observed MW:	50kDa

Synonyms:

ANXA7, ANX7, SNX, SYNEXIN

Background:

Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion.

UniProt Protein Function:	ANXA7: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Interacts with PDCD6. Isoform 1 is expressed in brain, heart and skeletal muscle. Isoform 2 is more abundant in liver, lung, kidney, spleen, fibroblasts and placenta. Belongs to the annexin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Calcium-binding; Motility/polarity/chemotaxis; Lipid-binding Chromosomal Location of Human Ortholog: 10q22.2 Cellular Component: endoplasmic reticulum membrane; membrane; plasma membrane; nuclear envelope; cytosol; nucleus Molecular Function:integrin binding; protein binding; calcium-dependent phospholipid binding; calcium ion binding; calcium-dependent protein binding Biological Process: cellular calcium ion homeostasis; regulation of cell shape; cell proliferation; cellular water homeostasis; epithelial cell differentiation; hemostasis; autophagy; social behavior; response to calcium ion; response to organic cyclic substance; response to salt stress
NCBI Summary:	Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion. [provided by RefSeq, Jul 2008]
UniProt Code:	P20073
NCBI GenInfo Identifier:	215274186
NCBI Gene ID:	310
NCBI Accession:	P20073.3
UniProt Secondary Accession:	P20073,Q5F2H3, Q5T0M6, Q5T0M7,
UniProt Related Accession:	P20073
Molecular Weight:	488
NCBI Full Name:	Annexin A7
NCBI Synonym Full Names:	annexin A7
NCBI Official Symbol:	ANXA7
NCBI Official Synonym Symbols:	SNX; ANX7; SYNEXIN
NCBI Protein Information:	annexin A7; annexin-7; annexin VII
UniProt Protein Name:	Annexin A7
UniProt Synonym Protein Names:	Annexin VII; Annexin-7; Synexin
UniProt Gene Name:	ANXA7
UniProt Entry Name:	ANXA7_HUMAN

	Western blot analysis of extracts of various cell lines, using ANXA7 antibody (CAB3733) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of U2OS cells using ANXA7 antibody (CAB3733) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

ANXA7 Antibody (PACO02061)

ANXA7 Antibody (PACO07757)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)