Anti-Ki67 Antibody (CAB16919)

SKU:
CAB16919
  • Cell Cycle Antibodies 1 Anti-Ki67 Antibody CAB16919
  • Cell Cycle Antibodies 1 Anti-Ki67 Antibody CAB16919
  • Cell Cycle Antibodies 1 Anti-Ki67 Antibody CAB16919
  • Cell Cycle Antibodies 1 Anti-Ki67 Antibody CAB16919
€99 - €249

Description

Technical Manual

Anti-Ki67 Antibody

Product Name: Anti-Ki67 Antibody
Product SKU: CAB16919
Size: 20uL, 50 uL
Host Species: Rabbit
Purification Method: Affinity purification
Isotype: IgG
Background:

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.

Immunogen: Recombinant protein of human Ki67
Sequence:

Email for sequence

Gene ID: 4288
Swiss Prot: P46013
Synonyms: MKI67; KIA; MIB-; MIB-1; PPP1R105; marker of proliferation Ki-67; Ki67
Calculated MW: 319kDa/358kDa
Observed MW: 358kDa
Reactivity: Human, Mouse, Rat
Application: WB IHC IF
Recommended Dilution: WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Storage Buffer: Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key: Western blotting Immunohistochemistry Immunofluorescence 
Positive Samples: NIH/3T3
Cellular Location:  

Product Images

Anti-Ki67 Antibody (CAB16919)

Western blot analysis of extracts of NIH/3T3 cells, using Ki67 antibody (CAB16919) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Anti-Ki67 Antibody (CAB16919)

Immunohistochemistry of paraffin-embedded human colon using Ki67 antibody (CAB16919) at dilution of 1:100 (40x lens).

Anti-Ki67 Antibody (CAB16919)

Immunofluorescence analysis of C6 cells using Ki67 Rabbit pAb (CAB16919) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Ki67 Antibody (CAB16919)

Immunofluorescence analysis of U-2 OS cells using Ki67 Rabbit pAb (CAB16919) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Uniprot Information

UniProt Protein Function: KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.
UniProt Protein Details:

Protein type:Nucleolus; Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q26.2

Cellular Component: chromosome, pericentric region; condensed chromosome; cytoplasm; membrane; nucleolus; nucleus

Molecular Function:ATP binding; protein binding; protein C-terminus binding

Biological Process: cell proliferation; DNA metabolic process; hyaluronan metabolic process; meiosis; organ regeneration; response to organic cyclic substance

NCBI Summary: This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
UniProt Code: P46013
NCBI GenInfo Identifier: 118572663
NCBI Gene ID: 4288
NCBI Accession: P46013.2
UniProt Secondary Accession: P46013,Q5VWH2,
UniProt Related Accession: P46013
Molecular Weight: 319,444 Da
NCBI Full Name: Antigen KI-67
NCBI Synonym Full Names: marker of proliferation Ki-67
NCBI Official Symbol: MKI67 
NCBI Official Synonym Symbols: KIA; MIB-; MIB-1; PPP1R105 
NCBI Protein Information: antigen KI-67
UniProt Protein Name: Antigen KI-67
UniProt Gene Name: MKI67 
UniProt Entry Name: KI67_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
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