Anti-ANTXR1 Antibody (CAB6525)

Antibody Name:	Anti-ANTXR1 Antibody
Antibody SKU:	CAB6525
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 84-333 of human ANTXR1 (NP_060623.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	NCI-H460, HeLa, Mouse ovary, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 84-333 of human ANTXR1 (NP_060623.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VFST RGTT LMKL TEDR EQIR QGLE ELQK VLPG GDTY MHEG FERA SEQI YYEN RQGY RTAS VIIA LTDG ELHE DLFF YSER EANR SRDL GAIV YCVG VKDF NETQ LARI ADSK DHVF PVND GFQA LQGI IHSI LKKS CIEI LAAE PSTI CAGE SFQV VVRG NGFR HARN VDRV LCSF KIND SVTL NEKP FSVE DTYL LCPA PILK EVGM KAAL QVSM NDGL SFIS SSVI ITTT HCSL HKIA SGPT TAAC ME
Gene ID:	84168
Uniprot:	Q9H6X2
Cellular Location:	Cell membrane, Cell projection, Single-pass type I membrane protein, filopodium membrane, lamellipodium membrane
Calculated MW:	33kDa/37kDa/39kDa/41kDa/59kDa/62kDa
Observed MW:	75kDa

Synonyms:

ANTXR1, ATR, GAPO, TEM8

Background:

This gene encodes a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. The encoded protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin, the causative agent of the disease, anthrax. The binding of the protective antigen (PA) component, of the tripartite anthrax toxin, to this receptor protein mediates delivery of toxin components to the cytosol of cells. Once inside the cell, the other two components of anthrax toxin, edema factor (EF) and lethal factor (LF) disrupt normal cellular processes. Three alternatively spliced variants that encode different protein isoforms have been described.

UniProt Protein Function:	ANTXR1: Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells. Defects in ANTXR1 are associated with susceptibility to hemangioma capillary infantile (HCI). HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. Belongs to the ATR family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Actin-binding; Receptor, misc. Chromosomal Location of Human Ortholog: 2p13.1 Cellular Component: filopodium membrane; cell surface; plasma membrane; integral to membrane; endosome membrane Molecular Function:collagen binding; actin filament binding; protein binding; transmembrane receptor activity; metal ion binding Biological Process: actin cytoskeleton reorganization; reproductive process; pathogenesis; signal transduction Disease: Gapo Syndrome; Hemangioma, Capillary Infantile
NCBI Summary:	This gene encodes a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. The encoded protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin, the causative agent of the disease, anthrax. The binding of the protective antigen (PA) component, of the tripartite anthrax toxin, to this receptor protein mediates delivery of toxin components to the cytosol of cells. Once inside the cell, the other two components of anthrax toxin, edema factor (EF) and lethal factor (LF) disrupt normal cellular processes. Three alternatively spliced variants that encode different protein isoforms have been described. [provided by RefSeq, Oct 2008]
UniProt Code:	Q9H6X2
NCBI GenInfo Identifier:	17366074
NCBI Gene ID:	84168
NCBI Accession:	Q9H6X2.2
UniProt Secondary Accession:	Q9H6X2,Q4ZFV6, Q53QD8, Q96P02, Q9NVP3, A8K7U8, J7K7G4 J7KF88,
UniProt Related Accession:	Q9H6X2
Molecular Weight:	564
NCBI Full Name:	Anthrax toxin receptor 1
NCBI Synonym Full Names:	anthrax toxin receptor 1
NCBI Official Symbol:	ANTXR1
NCBI Official Synonym Symbols:	ATR; GAPO; TEM8
NCBI Protein Information:	anthrax toxin receptor 1; 2310008J16Rik; 2810405N18Rik; tumor endothelial marker 8
UniProt Protein Name:	Anthrax toxin receptor 1
UniProt Synonym Protein Names:	Tumor endothelial marker 8
Protein Family:	Anthrax toxin receptor
UniProt Gene Name:	ANTXR1
UniProt Entry Name:	ANTR1_HUMAN

	Western blot analysis of extracts of various cell lines, using ANTXR1 antibody (CAB6525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of MCF7 cells using ANTXR1 antibody (CAB6525). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
ANTXR1 Antibody (PACO07747)	Human ANTXR1 (Anthrax toxin receptor 1) ELISA Kit (HUFI05453)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)