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APPL2 Rabbit Polyclonal Antibody (CAB14590)

SKU:
CAB14590
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:APPL2 Rabbit Polyclonal Antibody
SKU:CAB14590
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human APPL2 (NP_060641.2).
Sequence:MPAV DKLL LEEA LQDS PQTR SLLS VFEE DAGT LTDY TNQL LQAM QRVY GAQN EMCL ATQQ LSKQ LLAY EKQN FALG KGDE EVIS TLHY FSKV VDEL NLLH TELA KQLA DTMV LPII QFRE KDLT EVST LKDL FGLA SNEH DLSM AKYS RLPK KKEN EKVK TEVG KEVA AARR KQHL SSLQ YYCA LNAL QYRK QMAM MEPM IGFA HGQI NFFK KGAE MFSK RMDS FLSS VADM VQSI QVEL EAEA EKMR VSQQ ELLS VDES VYTP DSDV AAPQ INRN LIQK AGYL NLRN KTGL VTTT WERL
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:DIP13B; APPL2
Positive Sample:SH-SY5Y,HT-29,Mouse heart,Rat pancreas
Conjugate:Unconjugated
Cellular Localization:Early endosome membrane, Nucleus, Peripheral membrane protein.
Calculated MW:74kDa
Observed MW:74kDa

The protein encoded by this gene is one of two effectors of the small GTPase RAB5A/Rab5, which are involved in a signal transduction pathway. Both effectors contain an N-terminal Bin/Amphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain, and a C-terminal phosphotyrosine binding (PTB) domain, and they bind the Rab5 through the BAR domain. They are associated with endosomal membranes and can be translocated to the nucleus in response to the EGF stimulus. They interact with the NuRD/MeCP1 complex (nucleosome remodeling and deacetylase /methyl-CpG-binding protein 1 complex) and are required for efficient cell proliferation. A chromosomal aberration t(12;22)(q24.1;q13.3) involving this gene and the PSAP2 gene results in 22q13.3 deletion syndrome, also known as Phelan-McDermid syndrome.

Purification Method:Affinity purification
Gene ID:55198
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using APPL2 Rabbit pAb (CAB14590) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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