Anti-BCL6 Antibody (CAB7173)

Antibody Name:	Anti-BCL6 Antibody
Antibody SKU:	CAB7173
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human BCL6 (NP_001697.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	Raji

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human BCL6 (NP_001697.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASP ADSC IQFT RHAS DVLL NLNR LRSR DILT DVVI VVSR EQFR AHKT VLMA CSGL FYSI FTDQ LKCN LSVI NLDP EINP EGFC ILLD FMYT SRLN LREG NIMA VMAT AMYL QMEH VVDT CRKF IKAS EAEM VSAI KPPR EEFL NSRM LMPQ DIMA YRGR EVVE NNLP LRSA PGCE SRAF APSL YSGL STPP ASYS MYSH LPVS SLLF SDEE FRDV RMPV ANPF PKER ALPC DSAR PVPG EYSR PTLE VSPN VCHS NIYS PKET IPEE ARSD MHYS VAEG
Gene ID:	604
Uniprot:	P41182
Cellular Location:	Nucleus
Calculated MW:	72kDa/78kDa
Observed MW:	86KDa

Synonyms:

BCL6, BCL5, BCL6A, LAZ3, ZBTB27, ZNF51

Background:

The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.

UniProt Protein Function:	Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4+ T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.
NCBI Summary:	The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Aug 2015]
UniProt Code:	P41182
NCBI GenInfo Identifier:	728952
NCBI Gene ID:	604
NCBI Accession:	P41182.1
UniProt Secondary Accession:	P41182,A7E241, B8PSA7, D3DNV5,
UniProt Related Accession:	P41182
Molecular Weight:
NCBI Full Name:	B-cell lymphoma 6 protein
NCBI Synonym Full Names:	B-cell CLL/lymphoma 6
NCBI Official Symbol:	BCL6
NCBI Official Synonym Symbols:	BCL5; LAZ3; BCL6A; ZNF51; ZBTB27
NCBI Protein Information:	B-cell lymphoma 6 protein
UniProt Protein Name:	B-cell lymphoma 6 protein
UniProt Synonym Protein Names:	B-cell lymphoma 5 protein; BCL-5; Protein LAZ-3; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51
Protein Family:	BCL-6 corepressor
UniProt Gene Name:	BCL6
UniProt Entry Name:	BCL6_HUMAN

	Western blot analysis of extracts of Raji cells, using BCL6 antibody (CAB7173) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of A549 cells using BCL6 antibody (CAB7173).

Antibodies	ELISA Kits
BCL6 Antibody (PACO02009)	Human BCL6 (B-cell lymphoma 6 protein) ELISA Kit (HUFI07982)
BCL6 Antibody (PACO04925)	Bcl-6 Colorimetric Cell-Based ELISA Kit
Phospho-BCL6 (S333) Antibody (PACO06980)
BCL6 Antibody (PACO06981)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)