Anti-BDKRB1 Antibody (CAB1959)

Antibody Name:	Anti-BDKRB1 Antibody
Antibody SKU:	CAB1959
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BDKRB1 (NP_000701.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, A-431

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BDKRB1 (NP_000701.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MASS WPPL ELQS SNQS QLFP QNAT ACDN APEA WDLL HRVL PTFI ISIC FFGL LGNL FVLL VFLL PRRQ LNVA EIYL ANLA ASDL VFVL GLPF WAEN IWNQ
Gene ID:	623
Uniprot:	P46663
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	40kDa
Observed MW:	40kDa

Synonyms:

BDKRB1, B1BKR, B1R, BDKRB2, BKB1R, BKR1, BRADYB1

Background:

Bradykinin, a 9 aa peptide, is generated in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. Two types of G-protein coupled receptors have been found which bind bradykinin and mediate responses to these pathophysiologic conditions. The protein encoded by this gene is one of these receptors and is synthesized de novo following tissue injury. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	BKR1: This is a receptor for bradykinin. Could be a factor in chronic pain and inflammation. Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB1 sub-subfamily.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1 Chromosomal Location of Human Ortholog: 14q32.1-q32.2 Cellular Component: endoplasmic reticulum; integral to plasma membrane; plasma membrane Molecular Function:protein binding Biological Process: elevation of cytosolic calcium ion concentration
NCBI Summary:	Bradykinin, a 9 aa peptide, is generated in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. Two types of G-protein coupled receptors have been found which bind bradykinin and mediate responses to these pathophysiologic conditions. The protein encoded by this gene is one of these receptors and is synthesized de novo following tissue injury. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Code:	P46663
NCBI GenInfo Identifier:	85681914
NCBI Gene ID:	623
NCBI Accession:	P46663.3
UniProt Secondary Accession:	P46663,Q546S7, Q8N0Y8, A8K7F5,
UniProt Related Accession:	P46663
Molecular Weight:	40,495 Da
NCBI Full Name:	B1 bradykinin receptor
NCBI Synonym Full Names:	bradykinin receptor B1
NCBI Official Symbol:	BDKRB1
NCBI Official Synonym Symbols:	B1R; BKR1; B1BKR; BKB1R; BDKRB2; BRADYB1
NCBI Protein Information:	B1 bradykinin receptor
UniProt Protein Name:	B1 bradykinin receptor
Protein Family:	B1 bradykinin receptor
UniProt Gene Name:	BDKRB1
UniProt Entry Name:	BKRB1_HUMAN

	Western blot analysis of extracts of various cell lines, using BDKRB1 antibody (CAB1959) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of A549 cells using BDKRB1 Rabbit pAb (CAB1959) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of C6 cells using BDKRB1 Rabbit pAb (CAB1959) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using BDKRB1 Rabbit pAb (CAB1959) at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-BDKRB1 Antibody (CAB16777)

BDKRB1 Antibody (PACO04973)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)